Lee Eun-Ung, Lim Hyun-Chang, Hong Ji-Youn, Lee Jung-Seok, Jung Ui-Won, Choi Seong-Ho
Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul, Korea.
Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, Korea.
Clin Oral Implants Res. 2016 Nov;27(11):e91-e99. doi: 10.1111/clr.12568. Epub 2015 Feb 12.
This study compared the bone regenerative effects of a recombinant human bone morphogenetic protein 2 (rhBMP-2)-loaded collagen-based biphasic calcium phosphate composite (BCPC) and rhBMP-2-loaded biphasic calcium phosphate (BCP).
The in vitro release profiles of rhBMP-2-loaded BCP and BCPC were measured. The animal surgery was performed on ten rabbits. Four 8-mm-diameter circular calvarial defects were made and filled with BCP, BCPC, rhBMP-2-loaded BCP (BMP + BCP) and rhBMP-2-loaded BCPC (BMP + BCPC). The animals were euthanized either 2 or 8 weeks after surgery.
The initial burst release of rhBMP-2 was greater for BCP than for BCPC, and both presented a slow release pattern thereafter. In rabbit calvarial defects, the space maintaining capability and graft resorption of all experimental groups did not show statistical differences at 2 and 8 weeks. New bone formation in the rhBMP-2-loaded groups was greater than in the non-loaded groups at both weeks, but the amount of new bone was comparable between both rhBMP-2-loaded groups at both weeks. There was a distinct histologic difference between the BMP + BCP and BMP + BCPC groups at 2 weeks; the new bone formation occurred more in the intergranular spaces and the BCP-to-bone contact was greater in the BMP + BCPC group, but these differences were no longer discernible at 8 weeks.
BCP- and BCPC-loaded rhBMP-2 significantly improved bone regeneration and BCPC led to a dense network of new bone and bone particles during the early healing period. BCPC can therefore be considered as a promising candidate for carrying rhBMP-2.
本研究比较了负载重组人骨形态发生蛋白2（rhBMP - 2）的胶原基双相磷酸钙复合材料（BCPC）和负载rhBMP - 2的双相磷酸钙（BCP）的骨再生效果。
测定了负载rhBMP - 2的BCP和BCPC的体外释放曲线。对10只兔子进行了动物手术。制作了4个直径8毫米的圆形颅骨缺损，并分别用BCP、BCPC、负载rhBMP - 2的BCP（BMP + BCP）和负载rhBMP - 2的BCPC（BMP + BCPC）填充。在术后2周或8周对动物实施安乐死。
BCP中rhBMP - 2的初始突释比BCPC更大，且之后两者均呈现缓慢释放模式。在兔颅骨缺损中，所有实验组在2周和8周时的空间维持能力和移植物吸收情况均无统计学差异。在这两个时间点，负载rhBMP - 2组的新骨形成均多于未负载组，但两个负载rhBMP - 2组在这两个时间点的新骨量相当。在2周时，BMP + BCP组和BMP + BCPC组之间存在明显的组织学差异；新骨形成更多发生在粒间空间，且在BMP + BCPC组中BCP与骨的接触更大，但这些差异在8周时不再明显。
负载rhBMP - 2的BCP和BCPC显著改善了骨再生，且BCPC在愈合早期导致了密集的新骨和骨颗粒网络。因此，BCPC可被视为负载rhBMP - 2的有前景的候选材料。
