Department of Hepatobiliary Surgery, The Affiliated Hospital of Guangdong Medical College 524001 Zhanjiang, China.
Clinical Research Center, The Affiliated Hospital of Guangdong Medical College 524001 Zhanjiang, China.
Am J Cancer Res. 2015 Aug 15;5(9):2697-707. eCollection 2015.
Our previous study indicates microRNA-506 (miR-506) is downregulated in hepatocellular carcinoma (HCC). In the current study, we investigate the effects of miR-506 on proliferation, migration and invasion in HCC. We report that enforced expression of miR-506 inhibits proliferation, migration and invasion in vitro, and suppresses tumor growth in vivo. Conversely, suppression of miR-506 exhibits promoting effects on proliferation, migration and invasion in vitro, and on tumor growth in vivo. In addition, miR-506 binds to the 3'UTR of F-spondin 1(SPON1), and enforced expression of miR-506 decreases accumulation of SPON1. Moreover, enforced expression of SPON1 and suppression of SPON1 alleviates effects of miR-506 mimics and inhibitors on proliferation, migration and invasion in vitro, respectively. In conclusion, microRNA-506 regulates proliferation, migration and invasion in HCC by targeting SPON1.
我们之前的研究表明，微小 RNA-506（miR-506）在肝细胞癌（HCC）中下调。在本研究中，我们研究了 miR-506 对 HCC 增殖、迁移和侵袭的影响。我们报告说，miR-506 的强制表达抑制了体外的增殖、迁移和侵袭，并抑制了体内的肿瘤生长。相反，miR-506 的抑制对体外的增殖、迁移和侵袭以及体内的肿瘤生长表现出促进作用。此外，miR-506 与 F-spondin 1（SPON1）的 3'UTR 结合，miR-506 的强制表达减少了 SPON1 的积累。此外，SPON1 的强制表达和 SPON1 的抑制分别缓解了 miR-506 模拟物和抑制剂对体外增殖、迁移和侵袭的影响。总之，miR-506 通过靶向 SPON1 调节 HCC 中的增殖、迁移和侵袭。
