Harrison D J, Kharbanda R, Bishop D, McLelland L I, Hayes J D
Department of Pathology, University of Edinburgh, Medical School, UK.
Carcinogenesis. 1989 Jul;10(7):1257-60. doi: 10.1093/carcin/10.7.1257.
Glutathione S-transferase (GST) in man comprise at least four gene families. Three of these families give rise to cytosolic isoenzymes (alpha, mu and pi classes), whilst the remainder is membrane bound and has been called microsomal GST. These enzymes are implicated in tumourogenesis and both pi class GST and alpha class GST have been described in four cases of human renal cell carcinoma. Using specific polyclonal rabbit antisera we have demonstrated by immunohistochemistry that all 12 renal carcinomas studied contained GST pi. Most tumours also contained GST alpha, GST mu and microsomal GST isoenzymes but their distribution was heterogeneous and sometimes very focal. This heterogeneity of GST isoenzyme distribution within tumours has not been well documented previously, but is relevant to our understanding of the functions of GST, and to the interpretation of biochemical quantification experiments using tissue extracts.
人类谷胱甘肽S-转移酶（GST）至少由四个基因家族组成。其中三个家族产生胞质同工酶（α、μ和π类），而其余的则与膜结合，被称为微粒体GST。这些酶与肿瘤发生有关，在四例人类肾细胞癌中均已发现π类GST和α类GST。我们使用特异性兔多克隆抗血清，通过免疫组织化学证明，所研究的12例肾癌均含有GST π。大多数肿瘤还含有GST α、GST μ和微粒体GST同工酶，但其分布是异质性的，有时非常局限。肿瘤内GST同工酶分布的这种异质性以前尚未得到充分记录，但与我们对GST功能的理解以及对使用组织提取物进行的生化定量实验的解释有关。
