Sun Zhi-Jing, Zhu Lan, Lang Jing-He, Wang Zhao, Liang Shuo
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
Chin Med J (Engl). 2015 Dec 5;128(23):3191-6. doi: 10.4103/0366-6999.170262.
Pelvic organ prolapse (POP) is a major health problem in adult women that involves many factors. No proteomic analysis has been conducted exclusively in POP patients. This study aimed to identify the differential expression of proteins that may be involved in POP by proteomic analysis.
Samples of the uterosacral ligament (USL) were collected from five POP patients and five non-POP patients matched according to age, parity, and menopausal status and analyzed using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to verify the mRNA expression of proteins that showed differential expression in the proteomic analyses.
Proteins differentially expressed between POP and non-POP patients were detected. Eight proteins that were down-regulated in the POP group were identified by MALDI-TOF-MS. These proteins included electron transfer flavoprotein, apolipoprotein A-I, actin, transgelin, cofilin-1, cyclophilin A, myosin, and galectin-1, and their expression was verified by qRT-PCR.
Using comparative proteomics, we identified eight differentially expressed proteins (including four cytoskeleton proteins and three proteins related to apoptosis) in the USL that may be involved in apoptosis associated with the tissue effects in POP pathophysiology.
盆腔器官脱垂（POP）是成年女性面临的一个主要健康问题，涉及多种因素。此前尚未专门针对POP患者进行蛋白质组学分析。本研究旨在通过蛋白质组学分析确定可能与POP相关的蛋白质的差异表达。
从五名POP患者和五名根据年龄、产次和绝经状态匹配的非POP患者中采集子宫骶韧带（USL）样本，并使用二维电泳和基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）进行分析。采用定量实时聚合酶链反应（qRT-PCR）验证在蛋白质组分析中表现出差异表达的蛋白质的mRNA表达。
检测到POP患者和非POP患者之间差异表达的蛋白质。通过MALDI-TOF-MS鉴定出POP组中八种下调的蛋白质。这些蛋白质包括电子传递黄素蛋白、载脂蛋白A-I、肌动蛋白、转胶蛋白、丝切蛋白-1、亲环蛋白A、肌球蛋白和半乳糖凝集素-1，其表达通过qRT-PCR得到验证。
通过比较蛋白质组学，我们在USL中鉴定出八种差异表达的蛋白质（包括四种细胞骨架蛋白和三种与细胞凋亡相关的蛋白质），它们可能参与了与POP病理生理学中组织效应相关的细胞凋亡。
