Jiang Xiaolong, Shen Shan, Cadwell Cathryn R, Berens Philipp, Sinz Fabian, Ecker Alexander S, Patel Saumil, Tolias Andreas S
Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA.
Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA. Bernstein Centre for Computational Neuroscience, Tübingen, Germany. Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany. Werner Reichardt Center for Integrative Neuroscience and Institute of Theoretical Physics, University of Tübingen, Tübingen, Germany.
Science. 2015 Nov 27;350(6264):aac9462. doi: 10.1126/science.aac9462.
Since the work of Ramón y Cajal in the late 19th and early 20th centuries, neuroscientists have speculated that a complete understanding of neuronal cell types and their connections is key to explaining complex brain functions. However, a complete census of the constituent cell types and their wiring diagram in mature neocortex remains elusive. By combining octuple whole-cell recordings with an optimized avidin-biotin-peroxidase staining technique, we carried out a morphological and electrophysiological census of neuronal types in layers 1, 2/3, and 5 of mature neocortex and mapped the connectivity between more than 11,000 pairs of identified neurons. We categorized 15 types of interneurons, and each exhibited a characteristic pattern of connectivity with other interneuron types and pyramidal cells. The essential connectivity structure of the neocortical microcircuit could be captured by only a few connectivity motifs.
自19世纪末20世纪初拉蒙·伊·卡哈尔的研究工作以来，神经科学家们推测，全面了解神经元细胞类型及其连接是解释复杂脑功能的关键。然而，对成熟新皮层中组成细胞类型及其接线图进行全面普查仍然难以实现。通过将八通道全细胞记录与优化的抗生物素蛋白-生物素-过氧化物酶染色技术相结合，我们对成熟新皮层第1、2/3和5层的神经元类型进行了形态学和电生理学普查，并绘制了超过11000对已识别神经元之间的连接图谱。我们将15种中间神经元进行了分类，每种中间神经元都表现出与其他中间神经元类型和锥体细胞的独特连接模式。新皮层微电路的基本连接结构仅由少数几种连接基序就能捕捉到。
