Awaad Aziz
Department of Zoology, Faculty of Science, Sohag University, Sohag 82524, Egypt.
Acta Histochem. 2016 Jan;118(1):46-55. doi: 10.1016/j.acthis.2015.11.006. Epub 2015 Nov 21.
Fucose is a monosaccharide that plays several immunological roles. This study investigated the comparative biosynthesis and cellular biodistribution of fucose residues in some tissues of tetrapoda representatives using lectin histochemistry. In this study, the mouse was used as a representative for mammalian, pigeon for avian, lizard for reptilian, and toad for amphibians. The localization of the fucose residues was seen in several cell types of mice ileum, such as villi microfold (M) cells, goblet cells, some of intestinal crypts cells, and lamina propria cells. In other tetrapoda representatives, fucose was only seen in M cells of lizard ileum and some cells of villi lamina propria of pigeon, lizard, and toad. It was also observed in the pancreatic acinar cells of the mouse and some cell aggregations of pancreatic parenchyma of the lizard. Contrarily, it was not seen either in pigeon or in toad pancreases parenchyma. Spleen of all animals showed the fucose residues in some splenic cells in the red pulp only, barring the white pulp. The liver parenchyma of all tetrapoda representatives hadn't fucose residues. The fucose cellular biodistribution in some cells of tetrapoda representatives differed based on the cell type. In the mouse, it was highly seen in the apical cytoplasm of the villi M cells as well as in the cup-like part of goblet cells. In addition, it was seen as "rings" in the granule membranes of the Ulex europeaus agglutinin I (UEAI(+)) cells in the intestinal crypts cells. Furthermore, the UEAI(+) cells in the lamina propria showed fucose granules in their cytoplasm. There is no clear evidence about the relation between the cellular biosynthesis of fucose residues and mucosal immune cells. The role of fucose residues in the pancreatic acinar cells are not well understood and need further investigations. In this study, fucose residues were synthesized in several types of cells in the mouse ileum, spleen and pancreas as compared with other tetrapoda. The data obtained from this study can help us to get more information about the cellular biodistribution and synthesis of fucose residues in several animal species rather than mammalians.
岩藻糖是一种具有多种免疫作用的单糖。本研究利用凝集素组织化学方法，对四足动物代表的一些组织中岩藻糖残基的比较生物合成及细胞生物分布进行了研究。在本研究中，小鼠被用作哺乳动物的代表，鸽子作为鸟类代表，蜥蜴作为爬行动物代表，蟾蜍作为两栖动物代表。在小鼠回肠的几种细胞类型中可见岩藻糖残基的定位，如绒毛微褶（M）细胞、杯状细胞、一些肠隐窝细胞和固有层细胞。在其他四足动物代表中，仅在蜥蜴回肠的M细胞以及鸽子、蜥蜴和蟾蜍绒毛固有层的一些细胞中发现岩藻糖。在小鼠的胰腺腺泡细胞和蜥蜴胰腺实质的一些细胞聚集体中也观察到了岩藻糖。相反，在鸽子或蟾蜍的胰腺实质中均未发现岩藻糖。所有动物的脾脏仅在红髓的一些脾细胞中显示有岩藻糖残基，白髓中没有。所有四足动物代表的肝实质均没有岩藻糖残基。四足动物代表的一些细胞中岩藻糖的细胞生物分布因细胞类型而异。在小鼠中，岩藻糖在绒毛M细胞的顶端细胞质以及杯状细胞的杯状部分中高度可见。此外，在肠隐窝细胞中，欧洲荆豆凝集素I（UEAI(+)）细胞的颗粒膜中可见岩藻糖呈“环状”。此外，固有层中的UEAI(+)细胞在其细胞质中显示有岩藻糖颗粒。关于岩藻糖残基的细胞生物合成与黏膜免疫细胞之间的关系，目前尚无明确证据。岩藻糖残基在胰腺腺泡细胞中的作用尚不清楚，需要进一步研究。在本研究中，与其他四足动物相比，小鼠回肠、脾脏和胰腺中的几种细胞类型均可合成岩藻糖残基。本研究获得的数据有助于我们了解几种动物物种而非仅哺乳动物中岩藻糖残基的细胞生物分布和合成情况。
