在大肠杆菌K-12中使用异柠檬酸脱氢酶结构基因进行e14的附着。
Use of the isocitrate dehydrogenase structural gene for attachment of e14 in Escherichia coli K-12.
作者信息
Hill C W, Gray J A, Brody H
机构信息
Department of Biological Chemistry, Milton S. Hershey Medical Center, Pennsylvania 17033.
出版信息
J Bacteriol. 1989 Jul;171(7):4083-4. doi: 10.1128/jb.171.7.4083-4084.1989.
Abstract
The e14 element appears to be integrated into the Escherichia coli K-12 isocitrate dehydrogenase structural gene (icd). In being integrated, it replaced the last 52 codons of the gene with a closely related sequence. The two versions of the icd gene produce proteins of the same length but differ by 12 base substitutions that would cause two conservative amino acid replacements.
摘要
e14元件似乎整合到了大肠杆菌K-12异柠檬酸脱氢酶结构基因（icd）中。在整合过程中，它用一个密切相关的序列取代了该基因的最后52个密码子。icd基因的这两个版本产生的蛋白质长度相同，但有12个碱基替换，这将导致两个保守的氨基酸替换。
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