Goodman A D
Oral Surg Oral Med Oral Pathol. 1977 May;43(5):766-70. doi: 10.1016/0030-4220(77)90064-0.
The purpose of this study was to develop a clinically practical technique for isolating predominant anaerobic microorganisms from the root canal systems of intact necrotic teeth. Fifty-five consecutive teeth, all of which were necrotic, were sampled for this study. The technique developed involved a transport solution into which each sample was placed. At least one anaerobic bacterium was isolated from each case, with as many as four anaerobic bacteria being isolated from one case. Over half of the total number of organisms isolated were anaerobes, and almost half of these organisms were gram-negative. Whereas this technique has enabled the author to isolate and identify the same spectrum of predominant anaerobic bacteria as has been reported recently by others using the rigid VPI technique, the author suggests that the technique described here be utilized when the VPI technique cannot be performed.
本研究的目的是开发一种临床实用技术，用于从完整坏死牙的根管系统中分离出主要的厌氧微生物。本研究对55颗连续的坏死牙进行了采样。所开发的技术涉及一种运送液，每个样本都置于该运送液中。每个病例至少分离出一种厌氧菌，有一个病例分离出多达四种厌氧菌。分离出的微生物总数中超过一半是厌氧菌，且这些微生物中近一半是革兰氏阴性菌。尽管该技术使作者能够分离和鉴定出与其他人最近使用严格的VPI技术所报告的相同种类的主要厌氧细菌，但作者建议，当无法进行VPI技术时，可采用此处描述的技术。
