Le Goff A, Bunetel L, Mouton C, Bonnaure-Mallet M
Equipe de Biologie Buccale, Faculté d'Odontologie, Université de Rennes, France.
Oral Microbiol Immunol. 1997 Oct;12(5):318-22. doi: 10.1111/j.1399-302x.1997.tb00397.x.
This study aimed to evaluate the microbiota of necrotic pulp in teeth without carious lesions where the crown and root were intact and to test the sensitivity of this microbiota to antibiotics in order to improve treatment. The necrotic pulp was sampled from 26 single-rooted teeth in intact pulp chambers. A total of 84 strains were isolated. The number of species isolated per tooth varied from 2 to 8, with a strong component (81%) of anaerobic bacteria. The most commonly represented species were Bacteroides gracilis, Propionibacterium acnes, Fusobacterium nucleatum, Prevotella buccae and Eubacterium lentum. The sensitivity of these organisms to amoxicillin, amoxicillin combined with clavulanate and tetracycline was evaluated by Etest on 38 isolates. For all strains tested, the minimum inhibitory concentration values obtained were low and substantially below effective serum concentrations for these antibiotics. These data enable us to devise suitable treatments for acute development of apical lesions and to prevent dissemination of this source of infection to the rest of the host.
本研究旨在评估冠根完整且无龋损牙齿的坏死牙髓微生物群，并测试该微生物群对抗生素的敏感性，以改进治疗方法。从26颗牙髓腔完整的单根牙中采集坏死牙髓样本。共分离出84株菌株。每颗牙齿分离出的菌种数量从2种到8种不等，其中厌氧菌占很大比例（81%）。最常见的菌种为纤细拟杆菌、痤疮丙酸杆菌、具核梭杆菌、颊普雷沃菌和迟缓真杆菌。通过Etest法对38株分离菌评估了这些微生物对阿莫西林、阿莫西林联合克拉维酸和四环素的敏感性。对于所有测试菌株，获得的最低抑菌浓度值较低，且大大低于这些抗生素的有效血清浓度。这些数据使我们能够为根尖病变的急性发展设计合适的治疗方法，并防止这种感染源传播到宿主的其他部位。
