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绵羊间充质基质细胞：用于骨软骨组织工程的形态学、表型和功能特性

Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering.

作者信息

Sanjurjo-Rodríguez Clara, Castro-Viñuelas Rocío, Hermida-Gómez Tamara, Fernández-Vázquez Tania, Fuentes-Boquete Isaac Manuel, de Toro-Santos Francisco Javier, Díaz-Prado Silvia María, Blanco-García Francisco Javier

机构信息

Cell Therapy and Regenerative Medicine Unit, Rheumatology Group, Institute of Biomedical Research of A Coruña (INIBIC), University Hospital Complex A Coruña (CHUAC), Galician Health Service (SERGAS), Department of Medicine, Faculty of Health Sciences, University of A Coruña, A Coruña, Spain.

Tissue Bioengineering and Cell Therapy Unit (GBTTC-CHUAC), Rheumatology Group, Institute of Biomedical Research of A Coruña (INIBIC), University Hospital Complex A Coruña (CHUAC), Galician Health Service (SERGAS), A Coruña, Spain.

出版信息

PLoS One. 2017 Jan 31;12(1):e0171231. doi: 10.1371/journal.pone.0171231. eCollection 2017.

DOI:10.1371/journal.pone.0171231
PMID:28141815
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5283731/
Abstract

INTRODUCTION

Knowledge of ovine mesenchymal stromal cells (oMSCs) is currently expanding. Tissue engineering combining scaffolding with oMSCs provides promising therapies for the treatment of osteochondral diseases.

PURPOSE

The aim was to isolate and characterize oMSCs from bone marrow aspirates (oBMSCs) and to assess their usefulness for osteochondral repair using β-tricalcium phosphate (bTCP) and type I collagen (Col I) scaffolds.

METHODS

Cells isolated from ovine bone marrow were characterized morphologically, phenotypically, and functionally. oBMSCs were cultured with osteogenic medium on bTCP and Col I scaffolds. The resulting constructs were evaluated by histology, immunohistochemistry and electron microscopy studies. Furthermore, oBMSCs were cultured on Col I scaffolds to develop an in vitro cartilage repair model that was assessed using a modified International Cartilage Research Society (ICRS) II scale.

RESULTS

oBMSCs presented morphology, surface marker pattern and multipotent capacities similar to those of human BMSCs. oBMSCs seeded on Col I gave rise to osteogenic neotissue. Assessment by the modified ICRS II scale revealed that fibrocartilage/hyaline cartilage was obtained in the in vitro repair model.

CONCLUSIONS

The isolated ovine cells were demonstrated to be oBMSCs. oBMSCs cultured on Col I sponges successfully synthesized osteochondral tissue. The data suggest that oBMSCs have potential for use in preclinical models prior to human clinical studies.

摘要

引言

目前对绵羊间充质基质细胞（oMSCs）的了解正在不断扩展。将支架与oMSCs相结合的组织工程为骨软骨疾病的治疗提供了有前景的疗法。

目的

旨在从骨髓抽吸物中分离并鉴定oMSCs（oBMSCs），并评估其在使用β-磷酸三钙（bTCP）和I型胶原（Col I）支架进行骨软骨修复中的效用。

方法

对从绵羊骨髓中分离出的细胞进行形态学、表型和功能鉴定。将oBMSCs在bTCP和Col I支架上与成骨培养基一起培养。通过组织学、免疫组织化学和电子显微镜研究对所得构建体进行评估。此外，将oBMSCs在Col I支架上培养以建立体外软骨修复模型，并使用改良的国际软骨研究学会（ICRS）II级标准进行评估。

结果

oBMSCs呈现出与人类BMSCs相似的形态、表面标志物模式和多能能力。接种在Col I上的oBMSCs形成了成骨性新组织。通过改良的ICRS II级标准评估发现，在体外修复模型中获得了纤维软骨/透明软骨。

结论

已证明分离出的绵羊细胞为oBMSCs。在Col I海绵上培养的oBMSCs成功合成了骨软骨组织。数据表明，oBMSCs在人类临床研究之前的临床前模型中有应用潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff1e/5283731/535f4ecf4903/pone.0171231.g009.jpg
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