Immunohistochemical analysis of non-Hodgkin's lymphomas with the monoclonal antibody to follicular center lymphocytes reactive in paraffin sections.

The monoclonal antibody designated LN-1 was used in an attempt to identify the antigen in follicular center cell lymphomas using tissue sections fixed in formalin. The LN-1 antibody has been shown in previous studies to identify follicular center cells and give reproducible results in tissue fixed in B5. We used the ABC peroxidase technique to examined formalin-fixed, paraffin embedded sections representing 52 cases of various histologic subgroups of non-Hodgkin's lymphomas based upon the Lukes-Collins classification. Following immunostaining with LN-1 using overnight incubation of the antibody and papain treated sections, 37 cases, and all of the 38 cases previously diagnosed as follicular center cell lymphomas, gave a positive reaction to the LN-1 monoclonal antibody.