Marder R J, Variakojis D, Silver J, Epstein A L
Lab Invest. 1985 May;52(5):497-504.
Monoclonal antibodies (MoAbs) to B cell- and T cell-specific antigens uniformly have been restricted in their use to cell suspension or frozen section techniques because the antigens that they identify are either masked or lost in the fixation or paraffin-embedding processes. Antiimmunoglobulin antisera, although readily identifying cytoplasmic immunoglobulin in paraffin sections, has not been as useful as originally hoped since the majority of B cell lymphomas express surface immunoglobulins, which requires cell suspensions or frozen sections for detection. Because cell suspension procedures disrupt tissue architecture and frozen section techniques grossly distort morphology, neither method allows the combination of optimal morphologic and immunologic classification of lymphomas. We recently reported two MoAbs, LN-1 and LN-2, that react with B cells in paraffin sections. LN-1 reacts with the surface membrane and cytoplasm of germinal center B cells. LN-2 reacts uniquely with the nuclear membrane and cytoplasm of mantle zone and germinal center B cells and interdigitating histiocytes. We have also identified a new MoAb, LN-3, that reacts with the HLA-DR antigen in paraffin sections. We now report the use of LN-1, LN-2, and LN-3 in the analysis of paraffin sections from 58 non-Hodgkin's lymphomas and 15 cases of Hodgkin's disease. The types of cells reactive with these MoAbs in neoplastic lymphoid proliferations largely recapitulate their benign morphologic and immunologic counterparts. As a panel, LN-1, LN-2, and LN-3 were reactive with 98% of B cell lymphomas, and LN-1 and LN-2 were negative on all T cell lymphomas. In addition to identifying the cell of origin of these malignant proliferations, these MoAbs were also useful for identifying architectural features in neoplastic lymph nodes. Thus, these reagents provide the ability to assess the immunologic phenotype of neoplastic lymphocytes in conjunction with the critical morphologic criteria requiring paraffin embedding.
针对B细胞和T细胞特异性抗原的单克隆抗体（MoAbs）一直局限于用于细胞悬液或冷冻切片技术，因为它们所识别的抗原在固定或石蜡包埋过程中要么被掩盖，要么丢失。抗免疫球蛋白抗血清虽然能在石蜡切片中轻易识别细胞质免疫球蛋白，但并未达到最初预期的效果，因为大多数B细胞淋巴瘤表达表面免疫球蛋白，而检测表面免疫球蛋白需要细胞悬液或冷冻切片。由于细胞悬液程序会破坏组织结构，冷冻切片技术会严重扭曲形态，这两种方法都无法实现淋巴瘤最佳形态学和免疫学分型的结合。我们最近报道了两种能与石蜡切片中的B细胞反应的单克隆抗体，LN-1和LN-2。LN-1与生发中心B细胞的表面膜和细胞质反应。LN-2独特地与套区和生发中心B细胞以及交错突组织细胞的核膜和细胞质反应。我们还鉴定出一种新的单克隆抗体LN-3，它能与石蜡切片中的HLA-DR抗原反应。我们现在报告使用LN-1、LN-2和LN-3对58例非霍奇金淋巴瘤和15例霍奇金病的石蜡切片进行分析。在肿瘤性淋巴样增殖中与这些单克隆抗体反应的细胞类型在很大程度上重现了它们良性的形态学和免疫学对应物。作为一组抗体，LN-1、LN-2和LN-3与98%的B细胞淋巴瘤反应，而LN-1和LN-2对所有T细胞淋巴瘤均呈阴性。除了识别这些恶性增殖的起源细胞外，这些单克隆抗体还可用于识别肿瘤性淋巴结中的结构特征。因此，这些试剂能够结合石蜡包埋所需的关键形态学标准来评估肿瘤淋巴细胞的免疫表型。
