Shu Lin-Hua, Lu Quan, Han Li-Ying, Dong Guang-Hui
Department of Pediatric Pulmonology, Shanghai Children's Hospital, Shanghai Jiao Tong University Shanghai 200062, China.
Department of Pediatrics, Shengjing Hospital of China Medical University Shenyang 110004, China.
Int J Clin Exp Pathol. 2015 Sep 1;8(9):11185-91. eCollection 2015.
The study was aimed to evaluate the potential biomarkers from pulmonary surfactant protein D (SP-D), Krebs von den Lungen-6 (KL-6), and 56-kD a human type I protein (HTI-56) in serum and bronchoalveolar lavage fluid samples of children with Mycoplasma pneumoniae pneumonia. This retrospective study, self-controlled study enrolled 34 Chinese children with M. pneumoniae pneumonia. The levels of SP-D, KL-6, and HTI-56 in bronchoalveolar lavage fluid samples were assessed and compared between patients with unilateral lung infection and contralateral lungs without any abnormal findings. Significant differences in the levels of SP-D, KL-6, and HTI-56 were observed in infected bronchoalveolar lavage fluid samples compared with uninfected samples (all P<0.05); however, there was no correlation between the serum level of SP-D, KL-6, and HTI-56 and their levels in infected and uninfected bronchoalveolar lavage fluid samples (P>0.05).
The high levels of SP-D, KL-6, and HTI-56 in infected bronchoalveolar lavage fluid samples may reflect the injury of alveolar epithelium caused by M. pneumoniae. Instead of SP-D in uninfected bronchoalveolar lavage fluid samples obtained by invasive bronchoscopy, serum SP-D may serve as a convenient medium to distinguish lung infection caused by M. pneumoniae.
本研究旨在评估肺炎支原体肺炎患儿血清和支气管肺泡灌洗液样本中肺表面活性蛋白D（SP-D）、克雷伯氏肺6（KL-6）和人I型蛋白56-kD（HTI-56）的潜在生物标志物。这项回顾性自我对照研究纳入了34名中国肺炎支原体肺炎患儿。评估并比较了单侧肺部感染患者与对侧无异常发现肺部的支气管肺泡灌洗液样本中SP-D、KL-6和HTI-56的水平。与未感染样本相比，感染的支气管肺泡灌洗液样本中SP-D、KL-6和HTI-56水平存在显著差异（均P<0.05）；然而，血清中SP-D、KL-6和HTI-56水平与感染和未感染的支气管肺泡灌洗液样本中的水平之间无相关性（P>0.05）。
感染的支气管肺泡灌洗液样本中SP-D、KL-6和HTI-56水平升高可能反映了肺炎支原体对肺泡上皮的损伤。血清SP-D而非通过侵入性支气管镜检查获得的未感染支气管肺泡灌洗液样本中的SP-D，可能是区分肺炎支原体引起的肺部感染的便捷介质。
