膜联蛋白A2在胃腺癌患者中的表达及意义与E-钙黏蛋白的关系
Expression and significance of annexin A2 in patients with gastric adenocarcinoma and the association with E-cadherin.
作者信息
Han Yuehua, Ye Jun, Dong Ying, Xu Zhipeng, DU Qin
机构信息
Department of Gastroenterology, Second Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China.
Department of Oncology, Second Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China.
出版信息
Exp Ther Med. 2015 Aug;10(2):549-554. doi: 10.3892/etm.2015.2565. Epub 2015 Jun 10.
Annexin A2 is a calcium-dependent phospholipid-binding protein, involved in invasion, angiogenesis and migration in cancer cells. The aims of the present study were to evaluate the expression levels of annexin A2 and E-cadherin in gastric adenocarcinoma (GAC), and to investigate the association between the expression of annexin A2 and that of E-cadherin and Ki67, in addition to various clinicopathological factors. This study included 126 patients that were histopathologically diagnosed with GAC. Tissue samples were acquired by surgical resection, and annexin A2 mRNA expression levels were determined using reverse transcription-quantitative polymerase chain reaction. Annexin A2, E-cadherin and Ki67 protein expression levels were detected using western blot analysis and/or immunohistochemical staining. The expression of annexin A2 mRNA and protein was significantly upregulated in the GAC tissues. Annexin A2 expression was detected in 52/126 cases (41.3%) of gastric cancer (GC), and correlations were identified between annexin A2 expression and Tumor, Node, Metastasis (TNM) stage (P=0.002), lymph node metastasis (P=0.016) and distal metastasis (P=0.005). The positive expression rates of E-cadherin and Ki67 in the tumor tissue of patients with GAC were 27.8% (35/126) and 56.2% (71/126), respectively. A negative correlation was observed between the expression of annexin A2 and E-cadherin (P<0.001). No significant association was detected between the expression levels of annexin A2 and Ki67 (P=0.801). In conclusion, upregulated annexin A2 expression was associated with lymph node metastasis, distal metastasis, advanced TNM stage and E-cadherin expression in patients with GAC. The association between the expression of annexin A2 and that of E-cadherin may indicate an underlying mechanism by which annexin A2 contributes to the metastasis in GC, and thus annexin A2 may represent a potential target for the treatment of GAC.
膜联蛋白A2是一种钙依赖性磷脂结合蛋白，参与癌细胞的侵袭、血管生成和迁移。本研究的目的是评估膜联蛋白A2和E-钙黏蛋白在胃腺癌（GAC）中的表达水平，并研究膜联蛋白A2的表达与E-钙黏蛋白、Ki67的表达以及各种临床病理因素之间的关联。本研究纳入了126例经组织病理学诊断为GAC的患者。通过手术切除获取组织样本，采用逆转录定量聚合酶链反应测定膜联蛋白A2 mRNA表达水平。使用蛋白质免疫印迹分析和/或免疫组织化学染色检测膜联蛋白A2、E-钙黏蛋白和Ki67蛋白表达水平。GAC组织中膜联蛋白A2 mRNA和蛋白的表达显著上调。在126例胃癌（GC）病例中有52例（41.3%）检测到膜联蛋白A2表达，并且膜联蛋白A2表达与肿瘤、淋巴结、转移（TNM）分期（P = 0.002）、淋巴结转移（P = 0.016）和远处转移（P = 0.005）之间存在相关性。GAC患者肿瘤组织中E-钙黏蛋白和Ki67的阳性表达率分别为27.8%（35/126）和56.2%（71/126）。膜联蛋白A2与E-钙黏蛋白的表达呈负相关（P < 0.001）。膜联蛋白A2和Ki67的表达水平之间未检测到显著关联（P = 0.801）。总之，膜联蛋白A2表达上调与GAC患者的淋巴结转移、远处转移、晚期TNM分期和E-钙黏蛋白表达相关。膜联蛋白A2与E-钙黏蛋白表达之间的关联可能表明膜联蛋白A2促进GC转移的潜在机制，因此膜联蛋白A2可能是GAC治疗的潜在靶点。
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