Salehi Maryam, Kalbasi Nader, Salehi Hassan, Kassaian Nazila, Salehi Marzieh, Khorvash Farzin, Salehi Mohamad Mehdi
The Research committee of Medical Students, Isfahan University of Medical Sciences, Isfahan, Iran.
Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Int J Prev Med. 2014 Dec;5(Suppl 3):S219-22.
Hepatitis G virus (HGV) is transmitted mainly by parenteral route and patients on maintenance hemodialysis (HD) are at risk for this infection. This study was conducted to estimate prevalence of infection through the presence of anti-HGV and to evaluate the clinical significance of HGV envelope protein E2 (anti-E2) in HD patients in compare with volunteer blood donors in Isfahan-Iran.
In a cross-sectional study, a total of 40 HD patients as cases and 40 healthy volunteer blood donors as negative controls were selected randomly in summer 2008. The epidemiological data were obtained in all subjects, and duration of HD was obtained in HD patients as well. All samples were tested for anti-E2 antibodies, hepatitis C virus (HCV)-antibody and hepatitis B virus surface antigen (HBs-Ag) by an enzyme-linked immunosorbent assay and a recombinant immunoblot assay was employed to confirm anti-HCV reactivity. Student's t-test, Chi-square test or Fisher exact test was used for data analysis and P < 0.05 was considered as statistically significant.
Ten of the 40 HD patients tested positive for anti-E2 (25%) and of 40 voluntary blood donors, 10 (5%) were positive for anti-E2 (P = 0.012). Anti-HCV antibodies and HBs-Ag were found in 4 and 1 HD patients, respectively. In anti-E2-positive patients, co-infection with HCV or hepatitis B virus was not significant. Furthermore, the mean duration of hemodialysis in anti-E2 positive and anti-E2 negative patients did not have significant differences.
HD patients are at increased risk of HGV infection in Isfahan-Iran. Since hepatitis G is a good predictor for parenteral transmission, it is suggested to test all of the blood for transfusion for HGV infection.
庚型肝炎病毒（HGV）主要通过肠道外途径传播，维持性血液透析（HD）患者有感染该病毒的风险。本研究旨在通过检测抗-HGV的存在来估计感染率，并与伊朗伊斯法罕的无偿献血者相比，评估HD患者中HGV包膜蛋白E2（抗-E2）的临床意义。
在一项横断面研究中，2008年夏季随机选取40例HD患者作为病例组，40例健康无偿献血者作为阴性对照组。获取所有受试者的流行病学数据，同时也获取HD患者的血液透析时长。所有样本均采用酶联免疫吸附测定法检测抗-E2抗体、丙型肝炎病毒（HCV）抗体和乙型肝炎病毒表面抗原（HBs-Ag），并采用重组免疫印迹法确认抗-HCV反应性。数据分析采用学生t检验、卡方检验或Fisher精确检验，P<0.05被认为具有统计学意义。
40例HD患者中有10例抗-E2检测呈阳性（25%），40例无偿献血者中有10例抗-E2呈阳性（5%）（P=0.012）。分别在4例和1例HD患者中检测到抗-HCV抗体和HBs-Ag。在抗-E2阳性患者中，与HCV或乙型肝炎病毒的合并感染并不显著。此外，抗-E2阳性和抗-E2阴性患者的平均血液透析时长没有显著差异。
在伊朗伊斯法罕，HD患者感染HGV的风险增加。由于庚型肝炎是肠道外传播的良好预测指标，建议对所有输血用血液进行HGV感染检测。
