Seyedmajidi Maryam, Shafaee Shahryar, Hashemipour Golnarsadat, Bijani Ali, Ehsani Hodis
Dental Materials Research Center, Dental Faculty, Babol University of Medical Sciences, Babol, Iran E-mail :
Asian Pac J Cancer Prev. 2015;16(17):7513-6. doi: 10.7314/apjcp.2015.16.17.7513.
Pyogenic granuloma is a common non-neoplastic connective tissue proliferation. ICAM-1 and VCAM-1 are vascular adhesion molecules and CD34 is a marker for evaluation of angiogenesis. The purpose of this study was to compare the immunohistochemical expression of ICAM-1, VCAM-1 and CD34 in oral pyogenic granuloma and normal gingiva.
This study was performed on thirty five formalin-fixed, paraffin embedded samples of gingival pyogenic granuloma. Also we used thirty five paraffined blocks of normal gingiva as control group which were taken from crown lengthening surgery. We employed immunohistochemistry staining for our prepared microscopic slides using monoclonal mouse anti-human antibodies against ICAM-1 (CD54), VCAM-1 (CD106) and CD34. Slides were examined under light microscope and then the mean amount of stained vessels also known as microvascular density (MVD) in highly vascularized areas (hot spots) was measured. Paired t-test and repeated measures ANOVA were used to compare the difference between quantitative variables and Chi-square test for qualitative variables in different groups. Pearson correlation coefficient was used to compare relations between quantitative variables. P<0.05 was considered significant.
The mean of MVD for ICAM-1, VCAM-1 and CD34 was significantly higher in pyogenic granuloma than normal gingiva (p<0.001 and p<0.001 and p<0.001, respectively). Expression of CD34 in pyogenic granuloma was significantly higher than ICAM-1 and VCAM-1 (P<0.001). Besides, expression of ICAM-1 in normal gingiva, was significantly lower than two other markers (p<0.001).
Regarding the results, it seems that ICAM-1, VCAM-1 and CD34 are useful biomarkers in evaluation of vascular and inflammatory lesions such as gingival pyogenic granuloma and the results indicate the role of these biomarkers in pathogenesis of oral pyogenic granuloma.
化脓性肉芽肿是一种常见的非肿瘤性结缔组织增生。细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）是血管黏附分子，而CD34是评估血管生成的标志物。本研究的目的是比较ICAM-1、VCAM-1和CD34在口腔化脓性肉芽肿和正常牙龈中的免疫组化表达。
本研究对35例经福尔马林固定、石蜡包埋的牙龈化脓性肉芽肿样本进行。我们还使用了35个来自牙冠延长术的正常牙龈石蜡块作为对照组。我们使用抗ICAM-1（CD54）、VCAM-1（CD106）和CD34的单克隆小鼠抗人抗体对制备的显微镜载玻片进行免疫组织化学染色。在光学显微镜下检查载玻片，然后测量高血管化区域（热点）中染色血管的平均数量，即微血管密度（MVD）。配对t检验和重复测量方差分析用于比较不同组定量变量之间的差异，卡方检验用于比较定性变量。Pearson相关系数用于比较定量变量之间的关系。P<0.05被认为具有统计学意义。
化脓性肉芽肿中ICAM-1、VCAM-1和CD34的MVD平均值显著高于正常牙龈（分别为p<0.001、p<0.001和p<0.001）。化脓性肉芽肿中CD34的表达显著高于ICAM-1和VCAM-1（P<0.001）。此外，正常牙龈中ICAM-1的表达显著低于其他两个标志物（p<0.001）。
基于这些结果，似乎ICAM-1、VCAM-1和CD34在评估血管和炎性病变如牙龈化脓性肉芽肿方面是有用的生物标志物，并且结果表明这些生物标志物在口腔化脓性肉芽肿发病机制中的作用。
