Lou Kai, Zhu Zhaohua, Zhang Hongmei, Wang Yanqing, Wang Xiaojiong, Cao Jian
Chemistry and Chemical Engineering, Nanjing University of Technology, Nanjing City, Jiangsu Province, 210009, People's Republic of China; Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province, 224002, People's Republic of China.
Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province, 224002, People's Republic of China.
Chem Biol Interact. 2016 Jan 5;243:54-61. doi: 10.1016/j.cbi.2015.11.020. Epub 2015 Nov 30.
Herein, the interaction between carboxylated multi-walled carbon nanotubes (MWCNTs-COOH) and bovine serum albumin has been investigated by using circular dichroism, UV-vis, and fluorescence spectroscopic methods and molecular modeling in order to better understand the basic behavior of carbon nanotubes in biological systems. The spectral results showed that MWCNTs-COOH bound to BSA and induced the relatively large changes in secondary structure of protein by mainly hydrophobic forces and π-π stacking interactions. Thermal denaturation of BSA in the presence of MWCNTs-COOH indicated that carbon nanotubes acted as a structure destabilizer for BSA. In addition, the putative binding site of MWCNTs-COOH on BSA was near to domain II. With regard to human health, the present study could provide a better understanding of the biological properties, cytotocicity of surface modified carbon nanotubes.
在此，通过圆二色性、紫外可见光谱和荧光光谱法以及分子模拟研究了羧基化多壁碳纳米管（MWCNTs-COOH）与牛血清白蛋白之间的相互作用，以便更好地了解碳纳米管在生物系统中的基本行为。光谱结果表明，MWCNTs-COOH与牛血清白蛋白结合，并主要通过疏水作用和π-π堆积相互作用诱导蛋白质二级结构发生较大变化。在MWCNTs-COOH存在下牛血清白蛋白的热变性表明，碳纳米管对牛血清白蛋白起到结构去稳定剂的作用。此外，MWCNTs-COOH在牛血清白蛋白上的假定结合位点靠近结构域II。关于人类健康，本研究可以更好地理解表面改性碳纳米管的生物学特性和细胞毒性。
