Fedina Veronika, Lavrova Daria, Dyachkova Tatyana, Pasko Anastasia, Zvonarev Anton, Panfilov Victor, Ponamoreva Olga, Alferov Sergey
Laboratory of Ecological and Medical Biotechnology, Tula State University, Friedrich Engels Street 157, 300012 Tula, Russia.
Biotechnology Department, Tula State University, Pr. Lenina 92, 300012 Tula, Russia.
Polymers (Basel). 2023 Mar 3;15(5):1296. doi: 10.3390/polym15051296.
The development of biofuel cells (BFCs) currently has high potential since these devices can be used as alternative energy sources. This work studies promising materials for biomaterial immobilization in bioelectrochemical devices based on a comparative analysis of the energy characteristics (generated potential, internal resistance, power) of biofuel cells. Bioanodes are formed by the immobilization of membrane-bound enzyme systems of VKM V-1280 bacteria containing pyrroloquinolinquinone-dependent dehydrogenases into hydrogels of polymer-based composites with carbon nanotubes. Natural and synthetic polymers are used as matrices, and multi-walled carbon nanotubes oxidized in hydrogen peroxide vapor (MWCNTox) are used as fillers. The intensity ratio of two characteristic peaks associated with the presence of atoms C in the sp and sp hybridization for the pristine and oxidized materials is 0.933 and 0.766, respectively. This proves a reduced degree of MWCNTox defectiveness compared to the pristine nanotubes. MWCNTox in the bioanode composites significantly improve the energy characteristics of the BFCs. Chitosan hydrogel in composition with MWCNTox is the most promising material for biocatalyst immobilization for the development of bioelectrochemical systems. The maximum power density was 1.39 × 10 W/mm, which is 2 times higher than the power of BFCs based on other polymer nanocomposites.
生物燃料电池（BFC）的发展目前具有很高的潜力，因为这些装置可以用作替代能源。这项工作基于对生物燃料电池的能量特性（产生的电位、内阻、功率）的比较分析，研究了用于生物电化学装置中生物材料固定化的有前景的材料。生物阳极是通过将含有吡咯并喹啉醌依赖性脱氢酶的VKM V - 1280细菌的膜结合酶系统固定到含碳纳米管的聚合物基复合材料的水凝胶中形成的。天然和合成聚合物用作基质，在过氧化氢蒸气中氧化的多壁碳纳米管（MWCNTox）用作填料。原始材料和氧化材料中与sp和sp²杂化中C原子存在相关的两个特征峰的强度比分别为0.933和0.766。这证明与原始纳米管相比，MWCNTox的缺陷程度降低。生物阳极复合材料中的MWCNTox显著改善了BFC的能量特性。壳聚糖水凝胶与MWCNTox组成的材料是用于生物电化学系统开发的生物催化剂固定化最有前景的材料。最大功率密度为1.39×10 W/mm，比基于其他聚合物纳米复合材料的BFC的功率高2倍。
