Legnani Sara, Pantchev Nikola, Forlani Annalisa, Zini Eric, Schares Gereon, Balzer Jörg, Roccabianca Paola, Ferri Filippo, Zanna Giordana
Studio Dermatologico Veterinario, Via G. Sismondi 62, 20133, Milan, Italy.
IDEXX Laboratories, Moerikestrasse 28/3, 71636, Ludwigsburg, Germany.
Vet Dermatol. 2016 Feb;27(1):49-e14. doi: 10.1111/vde.12273. Epub 2015 Dec 1.
Neosporosis is a multisystemic disease caused by the intracellular protozoan Neospora caninum. In dogs the disease primarily affects the central nervous system. Canine cutaneous neosporosis is a rare condition often associated with old age or concurrent immunosuppressive treatments for different underlying conditions.
A 10-year-old female spayed golden retriever dog affected by primary immune-mediated myelofibrosis and treated with immunosuppressive therapies for 6 weeks that developed severe cutaneous lesions.
Definitive diagnosis was based on several investigation techniques including serology (immunoblotting), immunohistochemistry (IHC), species-specific conventional and real-time PCR, and DNA sequencing.
Remission of cutaneous neosporosis was obtained with the administration of clindamycin while the concurrent immunosuppressive therapy was maintained to manage the underlying primary condition.
To the best of the authors' knowledge this is the first report of species-specific PCR and DNA sequencing used as diagnostic methods for canine cutaneous neosporosis emerging in a dog receiving immunosuppressive therapy.
新孢子虫病是一种由细胞内原生动物犬新孢子虫引起的多系统疾病。在犬类中，该疾病主要影响中枢神经系统。犬皮肤新孢子虫病是一种罕见病症，常与老龄或因不同基础疾病进行的并发免疫抑制治疗有关。
一只10岁绝育雌性金毛猎犬，患有原发性免疫介导性骨髓纤维化，接受了6周免疫抑制治疗，出现了严重的皮肤病变。
确诊基于多种检测技术，包括血清学（免疫印迹法）、免疫组织化学（IHC）、种属特异性常规和实时PCR以及DNA测序。
在维持并发免疫抑制治疗以控制潜在原发性疾病的同时，给予克林霉素后皮肤新孢子虫病得到缓解。
据作者所知，这是首次报道将种属特异性PCR和DNA测序用作接受免疫抑制治疗犬只中出现的犬皮肤新孢子虫病的诊断方法。
