CD163片段在猪繁殖与呼吸综合征病毒感染中的作用
Function of CD163 fragments in porcine reproductive and respiratory syndrome virus infection.
作者信息
Li Hong, Zhou En-Min, Liu Cheng-Qian, Yi Jian-Zhong
机构信息
Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences Shanghai 201106, China.
Veterinary Immunology Institute of Northwest A & F University, College of Veterinary Medicine, Northwest A & F University Yangling 712100, Shaanxi Province, China.
出版信息
Int J Clin Exp Med. 2015 Sep 15;8(9):15373-82. eCollection 2015.
Monocyte/macrophage scavenger receptor CD163 plays an important role in porcine reproductive and respiratory syndrome virus (PRRSV) infection. To identify the domains of CD163 involved in PRRSV infection, CD163 fragments P1 (1-798 bp), P2 (790-2046 bp), P3 (2023-3345 bp), Y-P1 (160-798 bp), Y-P2 (790-2046 bp) and Y-P3 (2143-3084 bp) were expressed by eukaryotic and prokaryotic expression systems, respectively. Infection experiments revealed that non-permissive BHK-21 cells transfected with pCD163 could be infected by PRRSV. However, cells with truncated CD163 (P1, P2, or P3) were not susceptible to PRRSV. Meanwhile, Y-P1, Y-P2, and Y-P3 were expressed in E. coli and antisera to these peptides were prepared in mice. A virus blocking test showed that Y-P2 protein and anti-Y-P2 mouse serum could block PRRSV infection in a dose-dependent manner, while Y-P3 protein could improve virus infection.
单核细胞/巨噬细胞清道夫受体CD163在猪繁殖与呼吸综合征病毒（PRRSV）感染中起重要作用。为了确定CD163中参与PRRSV感染的结构域，分别通过真核和原核表达系统表达了CD163片段P1（1 - 798 bp）、P2（790 - 2046 bp）、P3（2023 - 3345 bp）、Y - P1（160 - 798 bp）、Y - P2（790 - 2046 bp）和Y - P3（2143 - 3084 bp）。感染实验表明，用pCD163转染的非允许性BHK - 21细胞可被PRRSV感染。然而，具有截短型CD163（P1、P2或P3）的细胞对PRRSV不敏感。同时，Y - P1、Y - P2和Y - P3在大肠杆菌中表达，并在小鼠体内制备了针对这些肽段的抗血清。病毒阻断试验表明，Y - P2蛋白和抗Y - P2小鼠血清能够以剂量依赖的方式阻断PRRSV感染，而Y - P3蛋白可促进病毒感染。
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