Song Qingzhang, Li Hui, Shao Hua, Li Chunling, Lu Xiao
Department of Infectious Diseases, Laiwu City People's Hospital Laiwu, P. R. China.
Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University Qingdao, P. R. China.
Int J Clin Exp Med. 2015 Sep 15;8(9):15458-65. eCollection 2015.
The present study is to investigate the relationship between microRNA (miR)-365 expression and the levels of interleukin (IL)-6 mRNA and protein in patients with active tuberculosis. From June 2011 to June 2014, 48 patients with active pulmonary tuberculosis induced by Mycobacterium tuberculosis were included in the study. In addition, 23 healthy subjects were enrolled as control. Macrophages were collected by pulmonary alveolus lavage. In addition, serum and mononuclear cells were isolated from peripheral blood. The levels of miR-365 and IL-6 in macrophages, mononuclear cells and serum were determined using quantitative real-time polymerase chain reaction. The protein expression of IL-6 in macrophages and mononuclear cells was measured using Western blotting, while that in serum was detected by enzyme-linked immunoabsorbent assay. Expression of IL-6 mRNA and protein was significantly enhanced in patients with active pulmonary tuberculosis. Increase of IL-6 protein concentration in serum was probably due to the release of IL-6 protein from mononuclear cells in the blood. In addition, miR-365 levels were significantly lowered in patients with active pulmonary tuberculosis. Up-regulated IL-6 expression in macrophages, mononuclear cells and serum in patients with active pulmonary tuberculosis is related to the down-regulation of miR-365, suggesting that miR-365 may regulate the occurrence and immune responses of active pulmonary tuberculosis via IL-6.
本研究旨在探讨微小RNA（miR）-365表达与活动性肺结核患者白细胞介素（IL）-6 mRNA及蛋白水平之间的关系。2011年6月至2014年6月，48例由结核分枝杆菌引起的活动性肺结核患者纳入本研究。此外，23名健康受试者作为对照。通过肺泡灌洗收集巨噬细胞。另外，从外周血中分离血清和单核细胞。采用定量实时聚合酶链反应测定巨噬细胞、单核细胞和血清中miR-365和IL-6的水平。采用蛋白质印迹法检测巨噬细胞和单核细胞中IL-6的蛋白表达，而血清中的IL-6蛋白表达则通过酶联免疫吸附测定法检测。活动性肺结核患者IL-6 mRNA和蛋白表达显著增强。血清中IL-6蛋白浓度升高可能是由于血液中单核细胞释放IL-6蛋白所致。此外，活动性肺结核患者的miR-365水平显著降低。活动性肺结核患者巨噬细胞、单核细胞和血清中IL-6表达上调与miR-365下调有关，提示miR-365可能通过IL-6调节活动性肺结核的发生及免疫反应。
