Wu Xue-Zhi, Wang Kui-Peng, Song Hong-Jie, Xia Jian-Hua, Jiang Yan, Wang Yong-Lian
Department of Cardiac Surgery, Zhumadian Center Hospital Zhumadian 463000, Henan, China.
The First Affiliated Hospital of Henan University of TCM Zhengzhou 450000, Henan, China.
Int J Clin Exp Med. 2015 Sep 15;8(9):15556-62. eCollection 2015.
Emerging evidence has suggested that dysregulation of microRNA-27a-3p (miR-27a-3p) may contribute to tumor development and progression in various types of cancers. However, its role in esophageal cancer is still unknown. In the present study, miR-27a-3p was significantly increased in esophageal squamous cell carcinoma (ESCC) tissues and cell lines. In esophageal cancer Eca109 cells, ectopic overexpression of miR-27a-3p promoted cell proliferation, meanwhile, cell proliferation was reduced by miR-27a-3p inhibition. Further studies showed that down-regulated miR-27a-3p expression could induced cell cycle arrest at the G1/S transition. In exploring mechanisms underlying the promotive role, our results revealed that miR-27a-3p markedly inhibited the expression of F-box and WD repeat domain-containing 7 (FBXW7). FBXW7, a tumor suppressor, exhibited significantly inhibitory effect on Eca109 cell proliferation. Thus our observations suggested that miR-27a-3p functioned as a tumor suppressor by targeting FBXW7. These findings indicated that miR-27a-3p could be considered as a potential therapeutic strategy for ESCC therapy.
新出现的证据表明，微小RNA-27a-3p（miR-27a-3p）的失调可能在多种癌症的肿瘤发生和进展中发挥作用。然而，其在食管癌中的作用仍不清楚。在本研究中，miR-27a-3p在食管鳞状细胞癌（ESCC）组织和细胞系中显著上调。在食管癌Eca109细胞中，miR-27a-3p的异位过表达促进细胞增殖，同时，miR-27a-3p抑制可降低细胞增殖。进一步研究表明，miR-27a-3p表达下调可诱导细胞周期阻滞于G1/S期转换。在探索其促进作用的潜在机制时，我们的结果显示miR-27a-3p显著抑制含F盒和WD重复结构域7（FBXW7）的表达。FBXW7作为一种肿瘤抑制因子，对Eca109细胞增殖具有显著抑制作用。因此，我们的观察结果表明，miR-27a-3p通过靶向FBXW7发挥肿瘤抑制作用。这些发现表明，miR-27a-3p可被视为ESCC治疗的一种潜在治疗策略。
