Geng Zhiwei, Tong Xiaoyan, Jia Hongjuan
Department of Neurology, Daqing Oilfield General Hospital Daqing, Heilongjiang, P. R. China.
Int J Clin Exp Med. 2015 Sep 15;8(9):15700-7. eCollection 2015.
Non-freezing cold injury is an injury characterized by neuropathy, developing when patients expose to cold environments. Reactive oxygen species (ROS) has been shown as a contributing factor for the non-freezing cold nerve injury. However, the detailed connections between non-freezing cold nerve injury and ROS have not been described. In order to investigate the relationship between non-freezing cold nerve injury and reactive oxygen species, we study the effects of two cooling methods-the continuous cooling and the intermittent cooling with warming intervals-on rat sciatic nerves. Specifically, we assess the morphological changes and ROS production of the sciatic nerves underwent different cooling treatments. Our data shows both types of cooling methods cause nerve injury and ROS production. However, despite of identical cooling degree and duration, the sciatic nerves processed by intermittent cooling with warming intervals present more ROS production, severer reperfusion injury and pathological destructions than the sciatic nerves processed by continuous cooling. This result indicates reactive oxygen species, as a product of reperfusion, facilitates non-freezing cold nerve injury.
非冻伤性冷损伤是一种以神经病变为特征的损伤，当患者暴露于寒冷环境时就会发生。活性氧（ROS）已被证明是导致非冻伤性冷神经损伤的一个因素。然而，非冻伤性冷神经损伤与ROS之间的详细联系尚未得到描述。为了研究非冻伤性冷神经损伤与活性氧之间的关系，我们研究了两种冷却方法——持续冷却和有升温间隔的间歇性冷却——对大鼠坐骨神经的影响。具体而言，我们评估了经过不同冷却处理的坐骨神经的形态变化和ROS生成情况。我们的数据表明，两种冷却方法都会导致神经损伤和ROS生成。然而，尽管冷却程度和持续时间相同，但与持续冷却处理的坐骨神经相比，有升温间隔的间歇性冷却处理的坐骨神经产生的ROS更多，再灌注损伤更严重，病理破坏更严重。这一结果表明，作为再灌注产物的活性氧促进了非冻伤性冷神经损伤。
