Karami Kheirabad Maryam, Namavar Jahromi Bahia, Tamadon Amin, Ramezani Amin, Ahmadloo Somayeh, Sabet Sarvestan Fatemeh, Koohi-Hosseinabadi Omid
Department of Basic Sciences, Azad University, Gachsaran Branch, Gachsaran, Iran.
Infertility Research Center, Department of OB-GYN, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Int J Mol Cell Med. 2015 Summer;4(3):182-7.
The effects of chronic stress and glucocorticoids receptor antagonist (RU486) on expression of melanocortin 4 receptor (MC4R) mRNA in arcuate nucleus (ARC) of male rats were evaluated. In this study, adult male Sprague Dawley rats were placed into four groups (n=6/group); stress, RU486, stress/RU486, and control groups. In stress group, the rats were restrained, 1 h/day, for 12 days. In RU486 group, the rats were injected RU486 for 12 days. In stress/RU486 group, the rats were injected RU486 1 h before the stress process for 12 days. Relative expression of MC4R mRNA was determined using real-time PCR. Relative expression of MC4R mRNA in the stress group was higher than that of the control rats (P<0.05). Relative expressions of MC4R mRNA were not different between the stress, RU486 and stress/RU486 groups (P>0.05). Chronic restraint stress causes increase in mRNA expression of MC4R in ARC and blockade of glucocorticoid receptors has no effect on this up-regulation.
评估了慢性应激和糖皮质激素受体拮抗剂（RU486）对雄性大鼠弓状核（ARC）中黑皮质素4受体（MC4R）mRNA表达的影响。在本研究中，成年雄性Sprague Dawley大鼠被分为四组（每组n = 6）：应激组、RU486组、应激/RU486组和对照组。应激组大鼠每天被束缚1小时，持续12天。RU486组大鼠注射RU486，持续12天。应激/RU486组大鼠在应激过程前1小时注射RU486，持续12天。使用实时PCR测定MC4R mRNA的相对表达。应激组中MC4R mRNA的相对表达高于对照大鼠（P<0.05）。应激组、RU486组和应激/RU486组之间MC4R mRNA的相对表达无差异（P>0.05）。慢性束缚应激导致ARC中MC4R的mRNA表达增加，糖皮质激素受体的阻断对此上调无影响。
