Sugiura M, Inagami T, Kon V
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
Biochem Biophys Res Commun. 1989 Jun 30;161(3):1220-7. doi: 10.1016/0006-291x(89)91372-7.
A marked increase in immunoreactive endothelin was observed in rat serum collected within 10-15 min after infusion of endotoxin. Endothelin level was 117 +/- 11.5 pg/ml (mean +/- S.E., N = 4) in rats exposed to endotoxin as compared with undetectable levels (less than 2 pg/ml, N = 4) in controls. We have also observed a significant stimulation of endothelin-release by endotoxin from cultured bovine transformed thoractic aortic endothelial cells at concentrations of endotoxin ranging between 0.1 and 10.0 micrograms/ml. Serum was indispensable for the stimulating effect of endotoxin, although serum itself did not show any effect at the concentration used (1%). These results suggest that endothelin plays an important role in mediation of pathophysiological responses caused by endotoxin. The levels of endothelin were measured by radioimmunoassay with high sensitivity.
在内毒素输注后10 - 15分钟内收集的大鼠血清中，观察到免疫反应性内皮素显著增加。与对照组（不可检测水平，低于2 pg/ml，N = 4）相比，暴露于内毒素的大鼠内皮素水平为117±11.5 pg/ml（平均值±标准误，N = 4）。我们还观察到，在内毒素浓度为0.1至10.0微克/毫升的范围内，培养的牛转化胸主动脉内皮细胞受内毒素刺激后内皮素释放显著增加。血清对于内毒素的刺激作用不可或缺，尽管血清本身在所使用的浓度（1%）下未显示任何作用。这些结果表明，内皮素在介导由内毒素引起的病理生理反应中起重要作用。内皮素水平通过高灵敏度放射免疫测定法测量。
