Nakamura T, Kasai K, Sekiguchi Y, Banba N, Takahashi K, Emoto T, Hattori Y, Shimoda S
Department of Endocrinology, Dokkyo University School of Medicine, Tochigi, Japan.
Eur J Pharmacol. 1991 Dec 3;205(3):277-82. doi: 10.1016/0014-2999(91)90910-i.
The effect of endotoxin on the release of endothelin, a novel potent vasoconstrictor peptide, was examined in anesthetized dogs and in cultured endothelial cells. Administration of 2.63 mg lipopolysaccharide, E. coli 0111:B4/kg body weight caused shock in the animals and produced a long-lasting increase in the plasma immunoreactive endothelin-1 level that remained higher than the basal level (1.83 pg/ml as mean level) from 30 to 120 min after the injection, with a peak at 90 min (8.15 pg/ml as mean level). In vitro immunoreactive endothelin-1 in a culture medium, in which calf pulmonary artery endothelial cells were incubated in the presence of 10% fetal bovine serum, increased dose dependently with the concentration of added lipopolysaccharide between 0.01 and 10 micrograms/ml. These data indicate that plasma endothelin increases during endotoxin shock and that stimulation by endotoxin, per se, in the presence of serum participates at least partially in the mechanism for its release.
在内毒素休克和培养的内皮细胞中，研究了内毒素对内皮素（一种新型强效血管收缩肽）释放的影响。给麻醉犬静脉注射2.63mg脂多糖（大肠杆菌0111:B4，每千克体重）可引起动物休克，并使血浆免疫反应性内皮素-1水平持久升高，注射后30至120分钟一直高于基础水平（平均水平为1.83pg/ml），90分钟时达到峰值（平均水平为8.15pg/ml）。在含有10%胎牛血清的培养基中培养小牛肺动脉内皮细胞，培养基中的免疫反应性内皮素-1随添加脂多糖浓度在0.01至10μg/ml之间呈剂量依赖性增加。这些数据表明，内毒素休克期间血浆内皮素增加，并且在内毒素存在下，血清本身的刺激至少部分参与了其释放机制。
