Department of Food, Agricultural and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH 44691-4096, USA.
Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA.
Bioresour Technol. 2016 Feb;201:50-7. doi: 10.1016/j.biortech.2015.11.035. Epub 2015 Nov 28.
The aim of this work was to isolate methanotrophs (methane oxidizing bacteria) that can directly convert biogas produced at a commercial anaerobic digestion (AD) facility to methanol. A methanotrophic bacterium was isolated from solid-state anaerobic digestate. The isolate had characteristics comparable to obligate methanotrophs from the genus Methylocaldum. This newly isolated methanotroph grew on biogas or purified CH4 and successfully converted biogas from AD to methanol. Methanol production was achieved using several methanol dehydrogenase (MDH) inhibitors and formate as an electron donor. The isolate also produced methanol using phosphate with no electron donor or using formate with no MDH inhibitor. The maximum methanol concentration (0.43±0.00gL(-1)) and 48-h CH4 to methanol conversion (25.5±1.1%) were achieved using biogas as substrate and a growth medium containing 50mM phosphate and 80mM formate.
本工作旨在分离能够直接将商业厌氧消化（AD）设施中产生的沼气转化为甲醇的甲烷氧化菌（甲烷氧化细菌）。从固态厌氧消化物中分离出一种甲烷氧化细菌。该分离株的特征与甲基营养菌属的专性甲烷氧化菌相当。这种新分离的甲烷氧化菌可以在沼气或纯化的 CH4 上生长，并成功地将 AD 产生的沼气转化为甲醇。甲醇的生产使用了几种甲醇脱氢酶（MDH）抑制剂和甲酸盐作为电子供体。该分离株还使用磷酸盐（无电子供体）或甲酸盐（无 MDH 抑制剂）生产甲醇。使用沼气作为底物和含有 50mM 磷酸盐和 80mM 甲酸盐的生长培养基，可获得最大甲醇浓度（0.43±0.00gL(-1)）和 48 小时 CH4 到甲醇的转化率（25.5±1.1%）。
