Meruvu Haritha, Wu Hui, Jiao Ziyue, Wang Liyan, Fei Qiang
School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi, China.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Synth Syst Biotechnol. 2020 Jun 26;5(3):173-178. doi: 10.1016/j.synbio.2020.06.007. eCollection 2020 Sep.
Methanotrophic bacteria are entities with innate biocatalytic potential to biofilter and oxidize methane into simpler compounds concomitantly conserving energy, which can contribute to copious industrial applications. The future and efficacy of such industrial applications relies upon acquiring and/or securing robust methanotrophs with taxonomic and phenotypic diversity. Despite several dramatic advances, isolation of robust methanotrophs is still a long-way challenging task with several lacunae to be filled in sequentially. Methanotrophs with high tolerance to methane can be isolated and cultivated by mimicking natural environs, and adopting strategies like adaptive metabolic evolution. This review summarizes existent and innovative methods for methanotrophic isolation and purification, and their respective applications. A comprehensive description of new insights shedding light upon how to isolate and concomitantly augment robust methanotrophic metabolism in an orchestrated fashion follows.
甲烷营养细菌是具有将甲烷生物过滤并氧化为更简单化合物同时保存能量的固有生物催化潜力的实体，这有助于大量的工业应用。此类工业应用的未来和功效依赖于获取和/或确保具有分类学和表型多样性的强大甲烷营养菌。尽管取得了一些重大进展，但分离强大的甲烷营养菌仍然是一项具有挑战性的长期任务，还有几个空白需要依次填补。通过模拟自然环境并采用适应性代谢进化等策略，可以分离和培养对甲烷具有高耐受性的甲烷营养菌。本综述总结了甲烷营养菌分离和纯化的现有方法和创新方法及其各自的应用。随后将全面描述关于如何以协调的方式分离并同时增强强大的甲烷营养菌代谢的新见解。
