Matsui Takahiro, Onouchi Takanori, Shiogama Kazuya, Mizutani Yasuyoshi, Inada Ken-Ichi, Yu Fuxun, Hayasaka Daisuke, Morita Koichi, Ogawa Hirohisa, Mahara Fumihiko, Tsutsumi Yutaka
Student, the Faculty of Medical Technology, Fujita Health University School of Health Sciences , Toyoake, Aichi, Japan ; Department of Pathology, Fujita Health University School of Medicine , Toyoake, Aichi, Japan ; Present address: Laboratory Medicine, Toyota Kosei Hospital , Toyota, Aichi, Japan.
Department of Pathology, Fujita Health University School of Medicine , Toyoake, Aichi, Japan.
Acta Histochem Cytochem. 2015 Oct 29;48(5):153-7. doi: 10.1267/ahc.15012. Epub 2015 Sep 15.
We performed pre-embedding electron microscopic study for visualizing the antigen and genome of severe fever with thrombocytopenia syndrome (SFTS) virus in the cytoplasm of macrophages of the human splenic red pulp, both requesting preheating treatment of sections. To pursue this, coated glass slides with unique characteristics are needed. Namely, during staining they must prevent detaching off sections, but after staining the sections must be transferred to epoxy resin. Aminopropyltriexoxysilane-coated glass slides, widely used for immunostaining, were resistant to transfer to epoxy resin. In contrast, coated glass slides designated as Thinlayer Advanced Cytology Assay System (TACAS) were suitable for this purpose. The technique is also applicable to the coated glass slide-requiring cytology practice, in which immunocytochemical evaluation is needed after cell transfer to another glass slide.
我们进行了包埋前电子显微镜研究，以观察人脾红髓巨噬细胞胞质中严重发热伴血小板减少综合征（SFTS）病毒的抗原和基因组，两者均需要对切片进行预热处理。为此，需要具有独特特性的包被载玻片。也就是说，在染色过程中它们必须防止切片脱落，但染色后切片必须转移到环氧树脂中。广泛用于免疫染色的氨丙基三乙氧基硅烷包被载玻片不耐转移到环氧树脂中。相比之下，指定为薄层高级细胞学检测系统（TACAS）的包被载玻片适用于此目的。该技术也适用于需要包被载玻片的细胞学实践，即在细胞转移到另一载玻片后需要进行免疫细胞化学评估的情况。
