Jiang Kai, Xue Yanfen, Ma Yanhe
State Key Laboratory of Microbial Resources and National Engineering Laboratory for Industrial Enzymes, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China ; University of Chinese Academy of Sciences, Beijing, China.
State Key Laboratory of Microbial Resources and National Engineering Laboratory for Industrial Enzymes, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Stand Genomic Sci. 2015 Dec 1;10:116. doi: 10.1186/s40793-015-0108-8. eCollection 2015.
Salinicoccus halodurans H3B36 is a moderately halophilic bacterium isolated from a sediment sample of Qaidam Basin at 3.2 m vertical depth. Strain H3B36 accumulate N (α)-acetyl-α-lysine as compatible solute against salinity and heat stresses and may have potential applications in industrial biotechnology. In this study, we sequenced the genome of strain H3B36 using single molecule, real-time sequencing technology on a PacBio RS II instrument. The complete genome of strain H3B36 was 2,778,379 bp and contained 2,853 protein-coding genes, 12 rRNA genes, and 61 tRNA genes with 58 tandem repeats, six minisatellite DNA sequences, 11 genome islands, and no CRISPR repeat region. Further analysis of epigenetic modifications revealed the presence of 11,000 m4C-type modified bases, 7,545 m6A-type modified bases, and 89,064 other modified bases. The data on the genome of this strain may provide an insight into the metabolism of N (α)-acetyl-α-lysine.
嗜盐盐球菌H3B36是从柴达木盆地垂直深度3.2米处的沉积物样本中分离出的中度嗜盐细菌。菌株H3B36积累N（α）-乙酰-α-赖氨酸作为抵御盐度和热应激的相容性溶质，在工业生物技术中可能具有潜在应用。在本研究中，我们使用PacBio RS II仪器上的单分子实时测序技术对菌株H3B36的基因组进行了测序。菌株H3B36的完整基因组为2,778,379 bp，包含2,853个蛋白质编码基因、12个rRNA基因和61个tRNA基因，有58个串联重复序列、6个微卫星DNA序列、11个基因组岛，且无CRISPR重复区域。对表观遗传修饰的进一步分析显示存在11,000个m4C型修饰碱基、7,545个m6A 型修饰碱基和89,064个其他修饰碱基。该菌株的基因组数据可能有助于深入了解N（α）-乙酰-α-赖氨酸的代谢。
