长白落叶松家系对干旱胁迫响应的生理评价及优良家系的蛋白质组学分析

Physiological evaluation of the responses of Larix olgensis families to drought stress and proteomic analysis of the superior family.

作者信息

Zhang L, Zhang H G, Pang Q Y

机构信息

State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China.

State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin, China

出版信息

Genet Mol Res. 2015 Dec 2;14(4):15577-86. doi: 10.4238/2015.December.1.9.

DOI:10.4238/2015.December.1.9

PMID:26634525

Abstract

The conifer Larix olgensis has been analyzed to delineate physiological and proteomic changes that occur under drought stress. Studies of the deleterious effects of drought in the larch families have mainly focused on photosynthesis. In the present study, when the intensity of drought was increased, plant height was inhibited as both POD and MDA levels increased, which indicates oxidative stress. Two-dimensional gel electrophoresis analysis detected 23 significantly differentially expressed proteins, of which 18 were analyzed by peptide mass fingerprinting by using MALDI-TOF/TOF. Eight spots were found to be up-regulated, while the other 10 spots were down-regulated during drought stress. The proteins that were induced by drought treatment have been implicated in the physiological changes that occurred. These results could provide additional information that could lead to a better understanding of the molecular basis of drought-sensitivity in larch plants.

摘要

对针叶树长白落叶松进行了分析，以描绘干旱胁迫下发生的生理和蛋白质组变化。落叶松家族中关于干旱有害影响的研究主要集中在光合作用上。在本研究中，当干旱强度增加时，株高受到抑制，同时过氧化物酶（POD）和丙二醛（MDA）水平升高，这表明存在氧化应激。二维凝胶电泳分析检测到23种显著差异表达的蛋白质，其中18种通过基质辅助激光解吸电离飞行时间串联质谱（MALDI-TOF/TOF）进行肽质量指纹图谱分析。发现8个斑点上调，而在干旱胁迫期间其他10个斑点下调。干旱处理诱导的蛋白质与所发生的生理变化有关。这些结果可以提供更多信息，从而有助于更好地理解落叶松植物干旱敏感性的分子基础。