Key Laboratory of Plants Adversity Adaptation and Genetic Improvement in Cold and Arid Regions of Inner Mongolia, Inner Mongolia Agricultural University, Hohhot 010018, China.
College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China.
Int J Mol Sci. 2024 Jun 21;25(13):6824. doi: 10.3390/ijms25136824.
Leaf senescence is essential for the growth and development of deciduous trees in the next season. , a deciduous coniferous tree, exhibits its most distinctive feature by turning yellow in the autumn and eventually shedding its leaves, resulting in significant changes in its appearance during the fall. Lysine acetylation plays an important role in diverse cellular processes; however, limited knowledge is available regarding acetylations in the needle senescence of In this study, the proteomics and acetylated modification omics of two phenotypic leaves, yellow and green (senescent and non-senescent) needles, were analyzed before autumn defoliation. In total, 5022 proteins and 4469 unique acetylation sites in 2414 lysine acylated proteins were identified, and this resulted in the discovery of 1335 differentially expressed proteins (DEPs) and 605 differentially expressed acetylated proteins (DAPs) in yellow versus green needles. There are significant differences between the proteome and acetylome; only 269 proteins were found to be DEP and DAP, of which 136 proteins were consistently expressed in both the DEP and DAP, 91 proteins were upregulated, and 45 proteins were down-regulated. The DEPs participate in the metabolism of starch and sucrose, while the DAPs are involved in glycolysis and the tricarboxylic acid cycle. Among them, DEPs underwent significant changes in glycolysis and citric acid cycling. Most of the enzymes involved in glycolysis and the citrate cycle were acetylated. DAPs were down-regulated in glycolysis and up-regulated in the citrate cycle. In all, the results of this study reveal the important role of lysine acetylation in the senescence of needles and provide a new perspective for understanding the molecular mechanism of leaf senescence and tree seasonal growth.
叶片衰老对于落叶树木在翌年的生长和发育至关重要。作为一种落叶针叶树，在秋季会变黄并最终落叶，其外观在秋季会发生显著变化，这是其最显著的特征。赖氨酸乙酰化在多种细胞过程中发挥着重要作用；然而，对于针叶树的针叶衰老过程中的乙酰化知之甚少。本研究在秋季落叶前，分析了两种表型叶片（黄色和绿色，衰老和非衰老）针叶的蛋白质组学和乙酰化修饰组学。共鉴定出 5022 种蛋白质和 2414 种赖氨酸酰化蛋白中的 4469 个独特乙酰化位点，从而发现黄色针叶与绿色针叶相比有 1335 个差异表达蛋白（DEPs）和 605 个差异表达乙酰化蛋白（DAPs）。蛋白质组学和乙酰化组学之间存在显著差异；仅发现 269 个蛋白是 DEP 和 DAP，其中 136 个蛋白在 DEP 和 DAP 中均有表达，91 个蛋白上调，45 个蛋白下调。DEPs 参与淀粉和蔗糖的代谢，而 DAPs 则参与糖酵解和三羧酸循环。其中，DEPs 在糖酵解和柠檬酸循环中发生显著变化。参与糖酵解和柠檬酸循环的大多数酶都发生了乙酰化。DAPs 在糖酵解中下调，在柠檬酸循环中上调。总之，本研究结果揭示了赖氨酸乙酰化在 针叶衰老过程中的重要作用，为理解叶片衰老和树木季节性生长的分子机制提供了新视角。
