Sipos J, McFarlane B M, McSorley C G, Gove C D, Williams R, McFarlane I G
Liver Unit, King's College Hospital, Denmark Hill, London, U.K.
J Pathol. 1989 Jul;158(3):247-52. doi: 10.1002/path.1711580313.
An immunohistochemical technique for the detection of the hepatic asialoglycoprotein receptor (ASGP-R) in cryostat sections of liver by polyclonal and monoclonal anti-ASGP-R antibodies is described. The procedure is based on the alkaline-phosphatase-avidin-biotin complex (ABC-AP) system and important features include fixation of the sections with periodate-lysine-paraformaldehyde (with or without dichromate) and an absolute requirement for blocking of endogenous biotin activity. The sensitivity of the technique is such that binding to ASGP-R can be detected with femtomolar concentrations of monoclonal anti-ASGP-R antibodies and, with polyclonal antisera, approaches that of a radioimmunoassay.
本文描述了一种免疫组织化学技术，用于通过多克隆和单克隆抗去唾液酸糖蛋白受体（ASGP-R）抗体检测肝脏冰冻切片中的肝去唾液酸糖蛋白受体（ASGP-R）。该方法基于碱性磷酸酶-抗生物素蛋白-生物素复合物（ABC-AP）系统，重要特征包括用高碘酸盐-赖氨酸-多聚甲醛（含或不含重铬酸盐）固定切片，以及绝对需要阻断内源性生物素活性。该技术的灵敏度使得可以用飞摩尔浓度的单克隆抗ASGP-R抗体检测与ASGP-R的结合，而使用多克隆抗血清时，其灵敏度接近放射免疫测定。
