López-de la Rosa Alberto, Martín-Montañez Vicente, López-Miguel Alberto, Calonge Margarita, Enríquez-de-Salamanca Amalia, González-García María Jesús
*MSc †PhD ‡MD Ocular Surface Group, IOBA-University of Valladolid, Campus Universitario Miguel Delibes, Valladolid, Spain (ALdlR, VM-M, AL-M, MC, AE-d-S, MJG-G); Networking Research Center on Bioengineering Biomaterials and Nanomedicine (CIBER-BBN), Valladolid, Spain (VM-M, MC, AE-d-S, MJG-G); and VISION I+D, SL, Edificio IOBA, Campus Universitario Miguel Delibes, Valladolid, Spain (AL-M).
Optom Vis Sci. 2016 Aug;93(8):892-900. doi: 10.1097/OPX.0000000000000784.
The aim of this study was to analyze whether symptoms of discomfort in hydrogel contact lens (HCL) wearers were associated with changes in corneal sensitivity or levels of tear inflammatory mediators.
Sixty-six subjects were included: 47 HCL wearers, further divided into 24 symptomatic and 23 asymptomatic wearers by the Contact Lens Dry Eye Questionnaire short form and 19 non-contact lens wearers. At least 24 h after HCL removal, we obtained scores from the Ocular Surface Disease Index and mechanical, hot, and cold corneal thresholds using a Belmonte esthesiometer. We collected 4 μl of tears with a capillary micropipette and measured levels of 12 inflammatory markers using a bead-based array: epidermal growth factor, fractalkine, interleukin-10 (IL-10), IL-1β, IL-1 receptor antagonist, IL-2, IL-4, IL-6, IL-8, monocyte chemoattractant protein 1, tumor necrosis factor-α, and matrix metalloproteinase 9.
There were no significant differences between groups in corneal sensitivity thresholds and levels of tear molecules. The following significant correlations were found in the total sample pooled: Ocular Surface Disease Index correlated with mechanical threshold (p < 0.01; rho = -0.324) and epidermal growth factor (p < 0.01, rho = -0.330), and mechanical threshold correlated with heat threshold (p < 0.01, rho = -0.321).
Twenty-four hours after HCL removal, symptoms of discomfort in HCL wearers are not related to changes in corneal sensitivity or tear inflammatory mediator levels. This might indicate either that HCL wear has no effect on these parameters or that ocular surfaces recover from HCL-caused effects during the first hours after HCL removal. However, there were correlations for associations between symptoms, corneal sensitivity, and some molecules in tears.
本研究旨在分析水凝胶隐形眼镜（HCL）佩戴者的不适症状是否与角膜敏感性变化或泪液炎症介质水平有关。
纳入66名受试者：47名HCL佩戴者，根据隐形眼镜干眼问卷简表进一步分为24名有症状佩戴者和23名无症状佩戴者，以及19名非隐形眼镜佩戴者。在摘除HCL至少24小时后，我们使用贝尔蒙特触觉计获得眼表疾病指数得分以及机械、热和冷角膜阈值。我们用毛细管微量移液器收集4微升泪液，并使用基于微珠的阵列测量12种炎症标志物的水平：表皮生长因子、 fractalkine、白细胞介素-10（IL-10）、IL-1β、IL-1受体拮抗剂、IL-2、IL-4、IL-6、IL-8、单核细胞趋化蛋白1、肿瘤坏死因子-α和基质金属蛋白酶9。
各组之间在角膜敏感性阈值和泪液分子水平上没有显著差异。在合并的总样本中发现了以下显著相关性：眼表疾病指数与机械阈值相关（p < 0.01；rho = -0.324）和表皮生长因子相关（p < 0.01，rho = -0.330），并且机械阈值与热阈值相关（p < 0.01，rho = -0.321）。
摘除HCL 24小时后，HCL佩戴者的不适症状与角膜敏感性或泪液炎症介质水平的变化无关。这可能表明HCL佩戴对这些参数没有影响，或者眼表在摘除HCL后的最初几个小时内从HCL引起 的影响中恢复。然而，症状、角膜敏感性和泪液中的一些分子之间存在相关性。
