Zhong Rui, Liu Hua, Wang Hong, Li Xiaojuan, He Zeng, Gangla Meiduo, Zhang Jingdan, Han Dingding, Liu Jiaxin
Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, Sichuan, China.
Tibet Autonomous Region blood center, Lhasa, Tibet, China.
PLoS One. 2015 Dec 4;10(12):e0144201. doi: 10.1371/journal.pone.0144201. eCollection 2015.
Hypoxia has been reported to cause the significant enhancement of hemoglobin (Hb) and hematocrit (Hct), which stabilizes at relatively high levels after an individual ascends to a high altitude. However, the quality of the suspended red blood cells (SRBCs) obtained from individuals at high altitudes such as Tibetan plateau migrants after storage has not been studied. In this study, we compared the storage quality of SRBCs prepared from Tibetan plateau and Deyang lowland populations by adding a normal volume of mannitol-adenine-phosphate (MAP), which is a common additive solution used in blood storage in Asian countries. The storage cell characteristics were examined on days 1, 7, 14 and 35.We found higher Hct and Hb levels and viscosity in the high altitude samples. The metabolic rates, including those for electrolytes and lactate, were higher in plateau SRBCs than in lowland SRBCs; these findings were consistent with the higher osmotic fragility and hemolysis of plateau SRBCs throughout the entire storage period. In addition, the reduction rates of 2,3-diphosphoglycerate (2,3-DPG) and oxygen tension to attain 50% oxygen saturation of Hb (P50) in plateau SRBCs were higher than those in lowland SRBCs, and the oxygen delivering capacity in plateau SRBCs was weaker than that in lowland SRBCs. We concluded that the storage quality of plateau SRBCs was inferior to that of lowland SRBCs when using the same concentration of MAP. We suggested that the optimal formula, including the MAP concentration or even a new additive solution, to store the plateau SRBCs must be assessed and regulated.
据报道，缺氧会导致血红蛋白（Hb）和血细胞比容（Hct）显著升高，个体登上高海拔地区后，这些指标会稳定在相对较高的水平。然而，从高海拔地区个体（如青藏高原移民）获取的悬浮红细胞（SRBCs）储存后的质量尚未得到研究。在本研究中，我们通过添加正常体积的甘露醇 - 腺嘌呤 - 磷酸盐（MAP）（这是亚洲国家血液储存中常用的添加剂溶液），比较了从青藏高原人群和德阳低地人群制备的SRBCs的储存质量。在第1、7、14和35天检查储存细胞的特征。我们发现高海拔样本中的Hct、Hb水平和粘度更高。高原SRBCs的代谢率，包括电解质和乳酸的代谢率，高于低地SRBCs；这些发现与高原SRBCs在整个储存期间较高的渗透脆性和溶血现象一致。此外，高原SRBCs中2,3 - 二磷酸甘油酸（2,3 - DPG）的降低率和使Hb达到50%氧饱和度（P50）时的氧张力降低率高于低地SRBCs，并且高原SRBCs的氧输送能力弱于低地SRBCs。我们得出结论，在使用相同浓度的MAP时，高原SRBCs的储存质量低于低地SRBCs。我们建议必须评估和调整用于储存高原SRBCs的最佳配方，包括MAP浓度甚至新的添加剂溶液。
