波长1060纳米、脉宽低于40飞秒的镱光纤激光器可增加对人体皮肤进行非线性光学显微镜检查时的穿透深度。
Sub-40 fs, 1060-nm Yb-fiber laser enhances penetration depth in nonlinear optical microscopy of human skin.
作者信息
Balu Mihaela, Saytashev Ilyas, Hou Jue, Dantus Marcos, Tromberg Bruce J
机构信息
University of California, Irvine, Beckman Laser Institute, Laser Microbeam and Medical Program, 1002 Health Sciences Road, Irvine, California 92612, United States.
Michigan State University, Department of Chemistry, 578 South Shaw Lane, East Lansing, Michigan 48824, United States.
出版信息
J Biomed Opt. 2015;20(12):120501. doi: 10.1117/1.JBO.20.12.120501.
Advancing the practical utility of nonlinear optical microscopy requires continued improvement in imaging depth and contrast. We evaluated second-harmonic generation (SHG) and third-harmonic generation images from ex vivo human skin and showed that a sub-40 fs, 1060-nm Yb-fiber laser can enhance SHG penetration depth by up to 80% compared to a >100 fs, 800 nm Ti:sapphire source. These results demonstrate the potential of fiber-based laser systems to address a key performance limitation related to nonlinear optical microscopy (NLOM) technology while providing a low-barrier-to-access alternative to Ti:sapphire sources that could help accelerate the movement of NLOM into clinical practice.
提高非线性光学显微镜的实际效用需要在成像深度和对比度方面持续改进。我们评估了来自离体人体皮肤的二次谐波产生（SHG）和三次谐波产生图像，结果表明，与大于100 fs、800 nm的钛宝石光源相比，一台低于40 fs、1060 nm的镱光纤激光器可将SHG的穿透深度提高多达80%。这些结果证明了基于光纤的激光系统在解决与非线性光学显微镜（NLOM）技术相关的关键性能限制方面的潜力，同时为钛宝石光源提供了一种易于使用的替代方案，这可能有助于加速NLOM进入临床实践。
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