Liao Yi-Hua, Kuo Wei-Cheng, Chou Sin-Yo, Tsai Cheng-Shiun, Lin Guan-Liang, Tsai Ming-Rung, Shih Yuan-Ta, Lee Gwo-Giun, Sun Chi-Kuang
Department of Dermatology, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei 10002, Taiwan ; Molecular Imaging Center, National Taiwan University, Taipei 10617, Taiwan ;
Department of Electrical Engineering, National Taiwan University, Taipei 10617, Taiwan.
Biomed Opt Express. 2014 Aug 28;5(9):3266-79. doi: 10.1364/BOE.5.003266. eCollection 2014 Sep 1.
Chronological skin aging is associated with flattening of the dermal-epidermal junction (DEJ), but to date no quantitative analysis focusing on the aging changes in the dermal papillae (DP) has been performed. The aim of the study is to determine the architectural changes and the collagen density related to chronological aging in the dermal papilla zone (DPZ) by in vivo harmonic generation microscopy (HGM) with a sub-femtoliter spatial resolution. We recruited 48 Asian subjects and obtained in vivo images on the sun-protected volar forearm. Six parameters were defined to quantify 3D morphological changes of the DPZ, which we analyzed both manually and computationally to study their correlation with age. The depth of DPZ, the average height of isolated DP, and the 3D interdigitation index decreased with age, while DP number density, DP volume, and the collagen density in DP remained constant over time. In vivo high-resolution HGM technology has uncovered chronological aging-related variations in DP, and sheds light on real-time quantitative skin fragility assessment and disease diagnostics based on collagen density and morphology.
自然皮肤老化与真皮-表皮交界处（DEJ）变平有关，但迄今为止，尚未对真皮乳头（DP）的老化变化进行定量分析。本研究的目的是通过具有亚飞升空间分辨率的体内谐波发生显微镜（HGM）来确定真皮乳头区（DPZ）中与自然老化相关的结构变化和胶原密度。我们招募了48名亚洲受试者，并在防晒的掌侧前臂上获取了体内图像。定义了六个参数来量化DPZ的三维形态变化，我们通过手动和计算方式对其进行分析，以研究它们与年龄的相关性。DPZ的深度、孤立DP的平均高度和三维交错指数随年龄增长而降低，而DP数量密度、DP体积和DP中的胶原密度随时间保持恒定。体内高分辨率HGM技术揭示了DP中与自然老化相关的变化，并为基于胶原密度和形态的实时定量皮肤脆性评估和疾病诊断提供了线索。
