Li Wenbo, Yang Xiaojun, Qian Lu, An Yulin, Fang Jie
a School of Life Sciences, Anhui University , Hefei , Anhui , China and.
b Plant Quarantine Laboratory of Jiangsu Entry-Exit Inspection and Quarantine Bureau of China , Nanjing , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4665-4667. doi: 10.3109/19401736.2015.1106493. Epub 2015 Dec 7.
In this paper, the complete mitochondrial genome of Anoplophora chinensis is sequenced and reported for the first time. The mitochondrial genome is a circular molecule of 15 805 bp, 13 protein-coding genes (PCGs), 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 control region. Nine protein-coding genes and 14 tRNA genes are encoded on the H strand, and the other four protein-coding genes and eight tRNA genes are encoded on the L strand. The arrangement of genes is identical to all know long-horn beetles mitochondrial genomes. The nucleotide composition of the A. chinensis mitogenome is strongly biased toward A + T nucleotides (77.65%). Finally, the phylogenetic relationships of 12 Lamiinae species were reconstructed based on nucleotide sequences of COI using the Bayesian inference method. These molecular-based phylogenies support the traditional morphologically based view of relationships within the Lamiinae.
本文首次对光肩星天牛的完整线粒体基因组进行了测序和报道。线粒体基因组是一个15805 bp的环状分子,包含13个蛋白质编码基因(PCGs)、22个转运RNA基因、2个核糖体RNA基因和1个控制区。9个蛋白质编码基因和14个tRNA基因在重链上编码,另外4个蛋白质编码基因和8个tRNA基因在轻链上编码。基因排列与所有已知的长角甲虫线粒体基因组相同。光肩星天牛线粒体基因组的核苷酸组成强烈偏向于A+T核苷酸(77.65%)。最后,使用贝叶斯推断方法,基于COI的核苷酸序列重建了12种沟胫天牛亚科物种的系统发育关系。这些基于分子的系统发育支持了基于传统形态学的沟胫天牛亚科内关系的观点。