马来西亚山羊口疮病毒的分离与系统发育分析
Isolation and phylogenetic analysis of caprine Orf virus in Malaysia.
作者信息
Abdullah Ashwaq Ahmed, Ismail Muhammad Farid Bin, Balakrishnan Krishnan Nair, Bala Jamilu Abubakar, Hani Homayoun, Abba Yusuf, Awang Isa Mohd Kamaruddin, Abdullah Faez Firdaus Jesse, Arshad Siti Suri, Nazariah Zeenatul Allaudin, Abdullah Rasedee, Mustapha Noordin Mohamed, Mohd-Lila Mohd-Azmi
机构信息
Institute of Bioscience, University Putra Malaysia, 43400 Serdang, Selangor DE Malaysia.
Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor DE Malaysia.
出版信息
Virusdisease. 2015 Dec;26(4):255-9. doi: 10.1007/s13337-015-0278-4. Epub 2015 Sep 21.
Orf virus is a DNA virus that causes contiguous ecthyma in goat and sheep. Infection of animals with this virus cause high mortality in young animals resulting in huge economic losses. In this study, we investigated an outbreak of Orf in a goat farm in Malaysia. Samples were collected from infected animals and viral isolation was done using both LT and MDCK cell lines. Molecular detection was done by conventional PCR for specific primers; B2L and F1L genes and phylogenetic analysis was done on the sequence data obtained. Cytopathic effects (CPE) were observed in both cell lines after 3 days of inoculation and were 50 % by the sixth day. PCR showed positive bands for both B2L and F1L genes and phylogenetic analysis showed that the Malaysian strain had close homology to the Chinese and Indian Orf virus isolates. This study gives more insight into the existing Orf viral strains in Malaysia and their relationship with other strains globally.
羊口疮病毒是一种DNA病毒，可引起山羊和绵羊的连续性脓疱性皮炎。动物感染这种病毒会导致幼畜高死亡率，从而造成巨大经济损失。在本研究中，我们调查了马来西亚一个山羊养殖场的羊口疮疫情。从感染动物身上采集样本，并使用LT和MDCK细胞系进行病毒分离。通过针对特异性引物B2L和F1L基因的常规PCR进行分子检测，并对获得的序列数据进行系统发育分析。接种3天后在两种细胞系中均观察到细胞病变效应（CPE），到第6天达到50%。PCR显示B2L和F1L基因均出现阳性条带，系统发育分析表明马来西亚毒株与中国和印度的羊口疮病毒分离株具有密切的同源性。本研究为深入了解马来西亚现有的羊口疮病毒株及其与全球其他毒株的关系提供了更多信息。
Zotero 插件