College of Animal Science and Veterinary Medicine, Jilin University, Changchun, China.
Vet Microbiol. 2010 May 19;142(3-4):408-15. doi: 10.1016/j.vetmic.2009.10.006. Epub 2009 Oct 31.
This study investigated an acute outbreak of contagious ecthyma dermatitis that occurred in November 2008 in a herd of 180 small-tailed Han sheep in the Jilin province of China. The pathological findings of this case revealed severe vascular proliferation, viral cytopathic changes in keratinocytes by vacuolar degeneration, ballooning degeneration, and eosinophilic cytoplasmic inclusions, suggesting that the disease could have been caused by an Orf virus (ORFV) infection. Immunohistochemistry, indirect immunofluorescence (IFA), and transmission electron microscopy supported the diagnosis of ORFV infection. Finally, the pathogen of the disease was identified using polymerase chain reaction (PCR) and sequences of major envelope protein genes (ORFV 011(B2L) and ORFV 059(F1L)). The full-length ORFV 011(B2L) and ORFV 059(F1L) genes were cloned and sequenced. The nucleotide and amino acid sequences of ORFV 011(B2L) and ORFV 059(F1L) genes in these outbreaks were analyzed, and their phylogenetic trees were constructed. The phylogenetic studies of ORFV 011(B2L) genes and ORFV 059(F1L) genes showed that the ORFV-Jilin province isolate clustered in different branches and was closer to the ORFV-Mukteswar 67/04 isolate and the ORFV-OV/C2 isolate, respectively. To date, there is no report on the molecular characterization of any Orf virus with other isolates around the world in mainland China. Further, the above results may provide some insight into the genotype of the etiological agent responsible for the contagious ecthyma dermatitis outbreak in the Jilin province, and could also provide a comparative view of the central coding region genomics of parapoxvirus (PPV).
本研究调查了 2008 年 11 月在中国吉林省一个 180 只小尾寒羊群体中爆发的传染性脓疱性皮炎。该病例的病理学发现揭示了严重的血管增生、角朊细胞中的病毒细胞病变，表现为空泡变性、气球样变性和嗜酸性细胞质包涵体，提示该病可能是由羊口疮病毒（ORFV）感染引起的。免疫组织化学、间接免疫荧光（IFA）和透射电子显微镜支持 ORFV 感染的诊断。最后，使用聚合酶链反应（PCR）和主要包膜蛋白基因（ORFV 011(B2L) 和 ORFV 059(F1L)）的序列鉴定了病原体。克隆并测序了全长 ORFV 011(B2L)和 ORFV 059(F1L)基因。分析了这些暴发中 ORFV 011(B2L)和 ORFV 059(F1L)基因的核苷酸和氨基酸序列，并构建了它们的系统发育树。ORFV 011(B2L)基因和 ORFV 059(F1L)基因的系统发育研究表明，ORFV-吉林省分离株聚类在不同分支中，分别与 ORFV-Mukteswar 67/04 分离株和 ORFV-OV/C2 分离株更接近。迄今为止，在中国大陆没有关于与世界其他地区任何其他分离株的 ORF 病毒的分子特征的报道。此外，上述结果可能为吉林省传染性脓疱性皮炎暴发的病因的基因型提供一些见解，并为副痘病毒（PPV）的中心编码区基因组学提供比较视角。
