Research and Development, MCI Santé Animale, Lot. 157, Z. I., Sud-Ouest (ERAC) B.P: 278, Mohammedia, 28810, Morocco; Hassan II Agronomic and Veterinary Institute, Rabat, Morocco.
Research and Development, MCI Santé Animale, Lot. 157, Z. I., Sud-Ouest (ERAC) B.P: 278, Mohammedia, 28810, Morocco.
Microb Pathog. 2024 Dec;197:107023. doi: 10.1016/j.micpath.2024.107023. Epub 2024 Oct 17.
Contagious ecthyma (CE), also known as ORF is a highly contagious zoonotic viral skin disease that affects humans, sheep, goats and other domesticated and wild animals. As reported here-in, the objective of this study was to investigate a suspected outbreak of both sheeppox and ORF diseases in a sheep herd during the winter of 2020 in Northwest Morocco. The affected sheep showed nodules and proliferative scabby skin lesions around the mouth and hairless area of the body. Samples of skin crust were collected for virus identification and isolation. A virus was isolated in Vero cells, lamb testis and heart cells and the cytopathic effect was characterized by cells aggregation, ballooning, and detachment. Initially, the suspensions of skin crust were positive for sheeppox virus (SPPV) by PCR. Subsequent testing of the isolated virus from skin crust of affected animals indicated that the virus was SPPV-negative and ORFV-positive by PCR. Furthermore, nucleotide sequences of the B2L aligned with reference ORFV isolates for genetic analysis. Phylogenetic analyses results confirmed that the isolated virus was ORFV and that the virus was closely related to ORFV strains isolated in Sudan and Malaysia. In conclusion, this study is the first reported detection of ORFV in Morocco, and therefore, poses as an imminent threat to the health of humans, domestic and wild animals.
接触传染性脓疱性皮炎（CE），也称为 ORF，是一种高度传染性的人畜共患病毒性皮肤病，影响人类、绵羊、山羊和其他家养和野生动物。如本报告所述，本研究的目的是调查 2020 年冬季摩洛哥西北部一个绵羊群中疑似发生的羊痘和 ORF 疾病爆发。受感染的绵羊在口部和无毛区域周围出现结节和增生性结痂皮肤病变。采集皮肤结痂样本进行病毒鉴定和分离。在 Vero 细胞、羔羊睾丸和心肌细胞中分离出一种病毒，其细胞病变特征为细胞聚集、气球样变和脱落。最初，皮肤结痂的悬浮液通过 PCR 对羊痘病毒（SPPV）呈阳性。随后对来自受影响动物皮肤结痂的分离病毒进行检测表明，PCR 结果为 SPPV 阴性和 ORFV 阳性。此外，B2L 的核苷酸序列与用于遗传分析的参考 ORFV 分离株对齐。系统发育分析结果证实，分离出的病毒为 ORFV，与在苏丹和马来西亚分离的 ORFV 株密切相关。总之，本研究首次在摩洛哥检测到 ORFV，因此对人类、家养和野生动物的健康构成迫在眉睫的威胁。
