Li Shan, Fei Guijun, Fang Xiucai, Yang Xilin, Sun Xiaohong, Qian Jiaming, Wood Jackie D, Ke Meiyun
Department of Gastroenterology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Department of Gastroenterology, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.
J Neurogastroenterol Motil. 2016 Apr 30;22(2):310-20. doi: 10.5056/jnm15082.
BACKGROUND/AIMS: Physical and/or emotional stresses are important factors in the exacerbation of symptoms in irritable bowel syndrome (IBS). Several lines of evidence support that a major impact of stress on the gastrointestinal tract occurs via the enteric nervous system. We aimed to evaluate histological changes in the submucosal plexus (SMP) and myenteric plexus (MP) of the distal ileum in concert with the intestinal motor function in a rat model of IBS with diarrhea.
The rat model was induced by heterotypic chronic and acute stress (CAS). The intestinal transit was measured by administering powdered carbon by gastric gavage. Double immunohistochemical fluorescence staining with whole-mount preparations of SMP and MP of enteric nervous system was used to assess changes in expression of choline acetyltransferase, vasoactive intestinal peptide, or nitric oxide synthase in relation to the pan neuronal marker, anti-Hu.
The intestinal transit ratio increased significantly from control values of 50.8% to 60.6% in the CAS group. The numbers of enteric ganglia and neurons in the SMP were increased in the CAS group. The proportions of choline acetyltransferase- and vasoactive intestinal peptide-immunoreactive neurons in the SMP were increased (82.1 ± 4.3% vs. 76.0 ± 5.0%, P = 0.021; 40.5 ± 5.9% vs 28.9 ± 3.7%, P = 0.001), while nitric oxide synthase-immunoreactive neurons in the MP were decreased compared with controls (23.3 ± 4.5% vs 32.4 ± 4.5%, P = 0.002).
These morphological changes in enteric neurons to CAS might contribute to the dysfunction in motility and secretion in IBS with diarrhea.
背景/目的：身体和/或情绪压力是肠易激综合征（IBS）症状加重的重要因素。多项证据支持，压力对胃肠道的主要影响是通过肠神经系统发生的。我们旨在评估腹泻型IBS大鼠模型中回肠末端黏膜下神经丛（SMP）和肌间神经丛（MP）的组织学变化以及肠道运动功能。
通过异型慢性和急性应激（CAS）诱导大鼠模型。通过胃内灌胃给予炭末来测量肠道转运。使用肠神经系统SMP和MP整装标本的双重免疫组织化学荧光染色来评估胆碱乙酰转移酶、血管活性肠肽或一氧化氮合酶表达相对于泛神经元标志物抗Hu的变化。
CAS组的肠道转运率从对照组的50.8%显著增加至60.6%。CAS组SMP中肠神经节和神经元数量增加。SMP中胆碱乙酰转移酶和血管活性肠肽免疫反应性神经元的比例增加（82.1±4.3%对76.0±5.0%，P=0.021；40.5±5.9%对28.9±3.7%，P=0.001），而与对照组相比，MP中一氧化氮合酶免疫反应性神经元减少（23.3±4.5%对32.4±4.5%，P=0.002）。
肠神经元对CAS的这些形态学变化可能导致腹泻型IBS的运动和分泌功能障碍。
