Schwieterman Alicia A, Steves Alyse N, Yee Vivian, Donelson Cory J, Bentley Melissa R, Santorella Elise M, Mehlenbacher Taylor V, Pital Aaron, Howard Austin M, Wilson Melissa R, Ereddia Danielle E, Effrein Kelsie S, McMurry Jonathan L, Ackley Brian D, Chisholm Andrew D, Hudson Martin L
Department of Molecular and Cellular Biology, Kennesaw State University, Kennesaw, Georgia 30144.
Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, California 95064.
Genetics. 2016 Feb;202(2):639-60. doi: 10.1534/genetics.115.185298. Epub 2015 Dec 8.
The Eph receptors and their cognate ephrin ligands play key roles in many aspects of nervous system development. These interactions typically occur within an individual tissue type, serving either to guide axons to their terminal targets or to define boundaries between the rhombomeres of the hindbrain. We have identified a novel role for the Caenorhabditis elegans ephrin EFN-4 in promoting primary neurite outgrowth in AIY interneurons and D-class motor neurons. Rescue experiments reveal that EFN-4 functions non-cell autonomously in the epidermis to promote primary neurite outgrowth. We also find that EFN-4 plays a role in promoting ectopic axon branching in a C. elegans model of X-linked Kallmann syndrome. In this context, EFN-4 functions non-cell autonomously in the body-wall muscle and in parallel with HS modification genes and HSPG core proteins. This is the first report of an epidermal ephrin providing a developmental cue to the nervous system.
Eph受体及其同源的ephrin配体在神经系统发育的许多方面发挥着关键作用。这些相互作用通常发生在单个组织类型内，要么引导轴突到达其终末靶点，要么界定后脑菱脑节之间的边界。我们已经确定了秀丽隐杆线虫ephrin EFN-4在促进AIY中间神经元和D类运动神经元的初级神经突生长方面的新作用。拯救实验表明，EFN-4在表皮中发挥非细胞自主性作用以促进初级神经突生长。我们还发现，在X连锁卡尔曼综合征的秀丽隐杆线虫模型中，EFN-4在促进异位轴突分支方面发挥作用。在这种情况下，EFN-4在体壁肌肉中发挥非细胞自主性作用，并与HS修饰基因和HSPG核心蛋白协同作用。这是关于表皮ephrin向神经系统提供发育线索的首次报道。
