人及不同动物血浆中戊糖基转移酶（EC 2.4.2.4）对[3H]胸腺嘧啶核苷的降解作用。
Degradation of [3H]thymidine by a pentosyltransferase (EC 2.4.2.4) in the plasma of man and different animals.
作者信息
Pauly J L, Schuller M G, Zelcer A A, Germain M J
出版信息
Experientia. 1977 May 15;33(5):668-70. doi: 10.1007/BF01946565.
PMID:266473
Abstract
[3H]Thymidine is degraded by an enzyme (thymidine phosphorylase; EC 2.4.2.4) which we have identified in the plasma of man and some animals. The presence of this enzyme in plasma or sera used to supplement culture media may, under certain experimental conditions, limit the validity of measuring the uptake of radiolabeled thymidine as a means of defining DNA synthesis.
摘要
[3H]胸苷可被一种酶（胸苷磷酸化酶；EC 2.4.2.4）降解，我们已在人和一些动物的血浆中鉴定出这种酶。在某些实验条件下，用于补充培养基的血浆或血清中存在这种酶，可能会限制将测量放射性标记胸苷的摄取作为定义DNA合成方法的有效性。
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