用于葡萄糖检测的非酶电化学传感器Co3O4超纳米片及Co3O4超纳米片-Ni(OH)2的合成与表征

Synthesis and characterization of Co3O4 ultra-nanosheets and Co3O4 ultra-nanosheet-Ni(OH)2 as non-enzymatic electrochemical sensors for glucose detection.

作者信息

Mahmoudian M R, Basirun W J, Woi Pei Meng, Sookhakian M, Yousefi Ramin, Ghadimi H, Alias Y

机构信息

Department of Chemistry, University of Malaya, Kuala Lumpur 50603, Malaysia; Department of Chemistry, Shahid Sherafat, University of Farhangian, 15916 Tehran, Iran.

Department of Chemistry, University of Malaya, Kuala Lumpur 50603, Malaysia.

出版信息

Mater Sci Eng C Mater Biol Appl. 2016 Feb;59:500-508. doi: 10.1016/j.msec.2015.10.055. Epub 2015 Oct 19.

DOI:10.1016/j.msec.2015.10.055

PMID:26652401

Abstract

The present study examines the synthesis of Co3O4 ultra-nanosheets (Co3O4 UNSs) and Co3O4 ultra-nanosheet-Ni(OH)2 (Co3O4 UNS-Ni(OH)2) via solvothermal process and their application as non-enzymatic electrochemical sensors for glucose detection. X-ray diffraction and transmission electron microscopy results confirmed the Co3O4 UNS deposition on Ni(OH)2 surface. The presence of Co3O4 UNSs on Ni (OH) 2 surface improved the sensitivity of glucose detection, from the increase of glucose oxidation peak current at the Co3O4 UNS-Ni(OH)2/glassy carbon electrode (current density: 2000μA·cm(-2)), compared to the Co3O4 UNSs. These results confirmed that Ni(OH)2 on glassy carbon electrode is a sensitive material for glucose detection, moreover the Co3O4 UNSs can increase the interaction and detection of glucose due to their high surface area. The estimated limit of detection (S/N=3) and limit of quantification (S/N=10) of the linear segment (5-40μM) are 1.08μM and 3.60μM respectively. The reproducibility experiments confirmed the feasibility of Co3O4 UNS-Ni(OH)2 for the quantitative detection of certain concentration ranges of glucose.

摘要

本研究通过溶剂热法研究了Co3O4超纳米片（Co3O4 UNSs）和Co3O4超纳米片-Ni(OH)2（Co3O4 UNS-Ni(OH)2）的合成及其作为非酶电化学传感器用于葡萄糖检测的应用。X射线衍射和透射电子显微镜结果证实了Co3O4 UNS沉积在Ni(OH)2表面。与Co3O4 UNSs相比，Co3O4 UNSs在Ni(OH)2表面的存在提高了葡萄糖检测的灵敏度，这是由于Co3O4 UNS-Ni(OH)2/玻碳电极上葡萄糖氧化峰电流的增加（电流密度：2000μA·cm(-2)）。这些结果证实，玻碳电极上的Ni(OH)2是一种用于葡萄糖检测的敏感材料，此外，Co3O4 UNSs因其高表面积可增加与葡萄糖的相互作用和检测。线性段（5-40μM）的估计检测限（S/N=3）和定量限（S/N=10）分别为1.08μM和3.60μM。重现性实验证实了Co3O4 UNS-Ni(OH)2用于定量检测一定浓度范围葡萄糖的可行性。