Tsujihashi H, Uejima S, Akiyama T, Kurita T
Department of Urology, Kinki University School of Medicine, Osaka, Japan.
Urol Int. 1989;44(1):5-9. doi: 10.1159/000281440.
Local immunocompetence in patients with bladder tumors was evaluated immunohistochemically using monoclonal antibodies. As well as investigating the subsets of peripheral blood lymphocytes by flow cytometry, the subpopulations of tissue-infiltrating lymphocytes were investigated in frozen specimens from bladder cancers (n = 25) and from noncancerous tissues (n = 4). Six serial sections were stained with Leu4, Leu7, Leu10, LeuM3, OKT4, and OKT8 antibodies, respectively. Marked lymphocytic infiltration into bladder tumors was a favorable prognostic sign. T cell infiltration was marked in the noninvasive group and was prominent around tumor cells, whereas B cells were rare in the stroma. T cells predominated over B cells in 19 of 25 bladder tumors. In patients with low-stage tumors, OKT8 cells were more prominent than OKT4 cells. NK cells accumulated around the cancer cells and infiltrated within cancer nests; NK cell infiltration was scanty in high-stage cases. There was a slight scattering of macrophages in 13 cases. In contrast to the findings in subsets of tissue-infiltrating lymphocytes, subsets of peripheral blood lymphocytes did not correlate with stage of disease. These findings suggest the presence of local immunosurveillance against bladder tumors. To elucidate host immune response against tumor, it may be important to characterize tissue-infiltrating lymphocytes.
采用单克隆抗体免疫组织化学方法评估膀胱肿瘤患者的局部免疫能力。除了通过流式细胞术研究外周血淋巴细胞亚群外，还对来自膀胱癌（n = 25）和非癌组织（n = 4）的冰冻标本中的组织浸润淋巴细胞亚群进行了研究。分别用Leu4、Leu7、Leu10、LeuM3、OKT4和OKT8抗体对连续的6个切片进行染色。膀胱肿瘤中明显的淋巴细胞浸润是一个良好的预后指标。在非侵袭性组中T细胞浸润明显，且在肿瘤细胞周围突出，而基质中B细胞很少。25例膀胱肿瘤中有19例T细胞多于B细胞。在低分期肿瘤患者中，OKT8细胞比OKT4细胞更突出。NK细胞聚集在癌细胞周围并浸润到癌巢内；在高分期病例中NK细胞浸润较少。13例中有轻微的巨噬细胞散在分布。与组织浸润淋巴细胞亚群的结果相反，外周血淋巴细胞亚群与疾病分期无关。这些发现提示存在针对膀胱肿瘤的局部免疫监视。为了阐明宿主对肿瘤的免疫反应，对组织浸润淋巴细胞进行特征性描述可能很重要。
