Song Yue, Zheng Jingjing, Yan Mingwei, Ding Weidong, Xu Kui, Fan Qingyu, Li Zhao
Department of Urologic and Pediatric Surgery, NO. 202 Hospital of People's Liberation Army, NO. 5 Guangrong Street, Shenyang, 110003, P.R.China.
Orthopedics Oncology Institute of Chinese People's Liberation Army and Department of Orthopedics, Tangdu Hospital, Fourth Military Medical University, NO. 1 Xinsi Road, Xi'an, 710038, P.R.China.
Sci Rep. 2015 Dec 10;5:18187. doi: 10.1038/srep18187.
Irreversible electroporation (IRE) is a novel ablation method that has been tested in humans with lung, prostate, kidney, liver, lymph node and presacral cancers. As a new non-thermal treatment, the use of IRE to ablate tumors in the musculoskeletal system might reduce the incidence of fractures. We aimed to determine the ablation threshold of cortical bone and to evaluate the medium- and long-term healing process and mechanical properties of the femur in a rabbit model post-IRE ablation. The ablation threshold of cortical bone was between 1090 V/cm and 1310 V/cm (120 pulses). IRE-ablated femurs displayed no detectable fracture but did exhibit signs of recovery, including osteoblast regeneration, angiogenesis and bone remodeling. In the ablation area, revascularization appeared at 4 weeks post-IRE. Osteogenic activity peaked 8 weeks post-IRE and remained high at 12 weeks. The mechanical strength decreased briefly 4 weeks post-IRE but returned to normal levels within 8 weeks. Our experiment revealed that IRE ablation preserved the structural integrity of the bone cortex, and the ablated bone was able to regenerate rapidly. IRE may hold unique promise for in situ bone tissue ablation because rapid revascularization and active osteogenesis in the IRE ablation area are possible.
不可逆电穿孔（IRE）是一种新型消融方法，已在患有肺癌、前列腺癌、肾癌、肝癌、淋巴结癌和骶前癌的人类患者中进行了测试。作为一种新的非热治疗方法，使用IRE消融肌肉骨骼系统中的肿瘤可能会降低骨折发生率。我们旨在确定皮质骨的消融阈值，并评估IRE消融后兔模型中股骨的中长期愈合过程和力学性能。皮质骨的消融阈值在1090 V/cm至1310 V/cm之间（120个脉冲）。IRE消融后的股骨未检测到骨折，但确实表现出恢复迹象，包括成骨细胞再生、血管生成和骨重塑。在消融区域，IRE后4周出现血管再形成。成骨活性在IRE后8周达到峰值，并在12周时保持较高水平。力学强度在IRE后4周短暂下降，但在8周内恢复到正常水平。我们的实验表明，IRE消融保留了骨皮质的结构完整性，消融后的骨能够快速再生。由于IRE消融区域可能实现快速血管再形成和活跃的成骨作用，IRE可能在原位骨组织消融方面具有独特的前景。
