Roma-Rodrigues Catarina, Alves-Barroco Cynthia, Raposo Luís R, Costa Mafalda N, Fortunato Elvira, Baptista Pedro Viana, Fernandes Alexandra R, Santos-Sanches Ilda
UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal.
UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal; CQE, Centro de Quimica Estrutural, Complexo 1, Instituto Superior Técnico, Universidade de Lisboa, Avenida Rovisco Pais, 1049-001 Lisboa, Portugal.
Microbes Infect. 2016 Apr;18(4):290-3. doi: 10.1016/j.micinf.2015.11.005. Epub 2015 Dec 1.
Streptococcus dysgalactiae subsp. dysgalactiae (SDSD) are considered exclusive animal pathogens; however, a putative zoonotic upper limb cellulitis, a prosthetic joint infection and an infective endocarditis were described in humans. To unravel if bovine SDSD isolates are able to infect human cells, the adherence and internalization to human primary keratinocytes of two bovine SDSD strains isolated from milk collected from udder were analyzed. Bacterial adhesion assays and confocal microscopy indicate a high adherence and internalization of SDSD isolates to human cells, suggesting for the first time the ability of bovine isolates to infect human cells.
乳房链球菌停乳亚种（SDSD）被认为是专性动物病原体；然而，在人类中曾有关于疑似人畜共患的上肢蜂窝织炎、人工关节感染和感染性心内膜炎的报道。为了弄清楚牛源SDSD分离株是否能够感染人类细胞，我们分析了从乳房采集的牛奶中分离出的两株牛源SDSD菌株对人原代表皮细胞的黏附及内化情况。细菌黏附试验和共聚焦显微镜检查表明，SDSD分离株对人类细胞具有高度的黏附及内化能力，这首次表明牛源分离株具有感染人类细胞的能力。
