脂肪酰化蛋白质的蛋白质组学分析。

Peng Tao, Thinon Emmanuelle, Hang Howard C

Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, NY 10065, United States.

Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, NY 10065, United States; The Crick Institute, 215 Euston Road, London NW1 2BE, United Kingdom.

Curr Opin Chem Biol. 2016 Feb;30:77-86. doi: 10.1016/j.cbpa.2015.11.008. Epub 2015 Dec 2.

Protein fatty-acylation in eukaryotes has been associated with many fundamental biological processes. However, the diversity, abundance and regulatory mechanisms of protein fatty-acylation in vivo remain to be explored. Herein, we review the proteomic analysis of fatty-acylated proteins, with a focus on N-myristoylation and S-palmitoylation. We then highlight major challenges and emerging methods for direct site identification, quantitation, and lipid structure characterization to understand the functions and regulatory mechanisms of fatty-acylated proteins in physiology and disease.

真核生物中的蛋白质脂肪酰化与许多基本生物学过程相关。然而，体内蛋白质脂肪酰化的多样性、丰度和调控机制仍有待探索。在此，我们综述了脂肪酰化蛋白质的蛋白质组学分析，重点关注N-肉豆蔻酰化和S-棕榈酰化。然后，我们强调了直接位点鉴定、定量和脂质结构表征的主要挑战及新兴方法，以了解脂肪酰化蛋白质在生理和疾病中的功能及调控机制。