Rahi Amid, Sattarahmady Naghmeh, Heli Hossein
Department of Nanomedicine, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Sci Rep. 2015 Dec 14;5:18060. doi: 10.1038/srep18060.
Gold nanoribbons covered by gold nanoblooms were sonoelectrodeposited on a polycrystalline gold surface at -1800 mV (vs. AgCl) with the assistance of ultrasound and co-occurrence of the hydrogen evolution reaction. The nanostructure, as a transducer, was utilized to immobilize a Brucella-specific probe and fabrication of a genosensor, and the process of immobilization and hybridization was detected by electrochemical methods, using methylene blue as a redox marker. The proposed method for detection of the complementary sequence, sequences with base-mismatched (one-, two- and three-base mismatches), and the sequence of non-complementary sequence was assayed. The fabricated genosensor was evaluated for the assay of the bacteria in the cultured and human samples without polymerase chain reactions (PCR). The genosensor could detect the complementary sequence with a calibration sensitivity of 0.40 μA dm(3) mol(-1), a linear concentration range of 10 zmol dm(-3) to 10 pmol dm(-3), and a detection limit of 1.71 zmol dm(-3).
在超声辅助以及析氢反应同时发生的条件下，于-1800 mV（相对于AgCl）的电位下，将覆盖有金纳米花的金纳米带声电沉积在多晶金表面上。该纳米结构作为换能器，用于固定布鲁氏菌特异性探针并制造基因传感器，固定和杂交过程通过电化学方法进行检测，使用亚甲基蓝作为氧化还原标记物。对所提出的检测互补序列、具有碱基错配（一、二和三个碱基错配）的序列以及非互补序列的方法进行了测定。所制造的基因传感器用于在无需聚合酶链反应（PCR）的情况下对培养样本和人类样本中的细菌进行测定。该基因传感器能够检测互补序列，校准灵敏度为0.40 μA dm(3) mol(-1)，线性浓度范围为10 zmol dm(-3)至10 pmol dm(-3)，检测限为1.71 zmol dm(-3)。
